Deshaun Watson was not charged by a second grand jury after it considered evidence of sexual assault and misconduct allegations.
The Cleveland Browns quarterback faces 22 civil lawsuits.
However, earlier in March, Harris County jurors did not find enough evidence for criminal proceedings, and Watson was again not indicted on Thursday after a Brazoria County grand jury also assessed the case.
"After a careful and thorough review of the facts and evidence documented in the reports prepared by the Brazoria County Sheriff's Office and the Houston Police Department, as well as hearing testimony from witnesses, the grand jury for Brazoria County has declined to charge Deshaun Watson with any crimes," Brazoria County District Attorney Tom Selleck said in a statement.
"Accordingly, this matter is closed."
Watson has strenuously denied the accusations since they emerged last year, overshadowing his attempts to push for a trade away from the Houston Texans.
He got his move to Cleveland after the first grand jury did not charge him.
Browns head coach Kevin Stefanski said last week: "Our organisation did a tremendous amount of background on Deshaun.
"We understand the concerns and questions that exist but are confident in the extensive work Andrew [Berry, executive vice-president of football operations and general manager] and his staff have done to feel confident about him joining our organisation."
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