The tourists currently find themselves 320-6 off 82 overs after winning the toss and batting first.
Chanderpaul led the way with 83 off 160 balls, hitting nine fours and a six in the process. The 26-year-old also made 83 in the first game.
Da Silva, the Captain, was much more proactive for his 82. His knock came off 94 balls and included 12 fours and a pair of sixes.
Alick Athanaze also chipped in with a well-compiled 59 off 66 balls including seven fours and two sixes while Reifer ended the day 56* off 90 balls including six fours and a six. Kevin Sinclair (22*) is currently partnering Reifer in the middle.
Nasum Ahmed has, so far, taken 3-92 off 26 overs for the hosts while Musfik Hasan has taken 2-50 in 13 overs.
West Indies “A” lead the series 1-0.
Afif Hossain and Saif Hassan have top-scored so far for the hosts with 37 and 31, respectively.
Akeem Jordan has taken 2-24 in his 11 overs, so far, while Kevin Sinclair, who has also bowled 11 overs, took 2-37.
The West Indies have made two changes to their XI from the first Test with Gudakesh Motie and Yannic Cariah being replaced by Keacy Carty and Anderson Phillip.
After winning the toss and choosing to bat, the tourists ended day one 220-2 off 68 overs.
McKenzie and Chanderpaul laid a solid foundation with a 130-run opening partnership.
McKenzie led the way with a 124-ball 86 including 12 boundaries and a six while Chanderpaul ended the day 70* off 190 balls including five fours.
Raymon Reifer was the other batsman dismissed for 26 while Alick Athanaze was 35* alongside Chanderpaul at stumps.
The Tigers took first strike after the Victorians won the toss and elected to field first, a decision that looked like the right one when Mahmudul Hasan Joy was dismissed by Pakistani pacer Naseem Shah in the third over with the score on 13.
That wicket brought Tigers skipper Shai Hope to the crease alongside Tamim Iqbal and the pair put on a T20 batting masterclass, blasting the Comilla bowling all over the Sylhet International Cricket Stadium.
A 184-run second wicket partnership between them helped the Tigers reach 197 after 19 overs before Iqbal was finally dismissed five runs short of a magnificent hundred. Iqbal hit 11 fours and four sixes in 61 deliveries.
In the end, Khulna ended their 20 overs on 210-2 with Hope ending not out on 91. His knock came off just 55 balls and included five fours and seven sixes.
The Victorians reply got off to the worst possible start when top batsman Litton Das was forced to retire hurt after being struck on the wrist with the second ball of the innings.
This brought Captain Imrul Kayes to the wicket and he and Mohammad Rizwan put on 22 before Kayes was dismissed for five in the third over.
Rizwan was then joined at the crease by Charles and the two put on a display like that of Hope and Iqbal in the Tigers’ innings.
The pair put on what turned into a match-winning 122-run partnership before Rizwan was dismissed for a 39-ball 73 in the 14th over.
Charles continued on his merry way and brought up a magnificent hundred with a six over cover in the 18th over. His knock came off 53 balls and included five fours and 10 sixes.
He closed proceedings with a six in the 19th over to help the Victorians complete the highest chase in BPL history.
The Victorians now have 12 points from nine games and have secured their spot in the playoffs with a game to spare.
Scores: Khulna Tigers 210-2 in 20 overs (Tamim Iqbal 95, Shai Hope 91*) Comilla Victorians 213-3 in 18.2 overs (Johnson Charles 107*, Mohammad Rizwan 73).
