New York Red Bulls captain Sean Nealis has vowed the team will learn from last week's defeat to the New England Revolution, when they were hamstrung by several absences.
The Red Bulls were beaten 1-0 in Foxborough following a run of four wins in five matches, with Lewis Morgan and Emil Forsberg among the big names missing due to international duty.
The result left them fourth in the Eastern Conference standings, six points adrift of Supporters' Shield frontrunners Inter Miami.
"We separated ourselves from the bottom half of the group and now we're kind of just lying there in the middle," centre-back Nealis said as he reflected on New York's last result.
"We'll learn from this. We'll get better from it. This is a young group out there.
"A lot got their first minutes of the season and I think we'll all learn how to get better and how to understand these environments and then get over the hump."
Nashville, meanwhile, sit ninth in the East, 10 points adrift of the Red Bulls.
They have taken four points from four games (one win, two draws, one defeat) since Rumba Munthali took over as interim head coach following the firing of Gary Smith.
They were also beaten by the Revs on their last MLS outing before the international break, and Munthali believes keeping players fresh will be key if they are to improve.
"It's a quick turnaround, and we have a lot of confidence in the group," Munthali said.
"We try to balance that, and we want to make sure guys are available for the whole season, not just game to game.
"It's about managing and making sure we're not straining guys and breaking them."
PLAYERS TO WATCH
New York Red Bulls – Elias Manoel
Having seen the likes of Morgan and Forsberg go on international duty, the Red Bulls could be left to rely on Manoel.
He has three goals and three assists in MLS play this term, despite only making eight starts. Only Morgan (nine) and Forsberg (six) have outscored him for New York.
Nashville SC – Hany Mukhtar
Mukhtar scored Nashville's only goal in their defeat to New England on June 1 from the penalty spot.
He has been directly involved in each of Nashville's last four MLS goals (two goals, two assists, with the last Nashville goal without a Mukhtar involvement coming on May 15.
MATCH PREDICTION – NEW YORK RED BULLS WIN
Nashville ended a seven-game run without an away victory by beating FC Cincinnati 2-0 on May 29, just their second win in their last 16 away league matches.
However, they may struggle on Saturday against a team unbeaten in 10 straight home games (seven wins, three draws), dating back to last year's playoffs.
The Red Bulls are also unbeaten in their last five meetings with Nashville, winning two and drawing three while only conceding once in that sequence.
OPTA WIN PROBABILITY
New York Red Bulls – 52%
Nashville SC – 23.5%
Draw – 24.5%