In his home stadium, Hudson-Smith, whose mother hails from Hanover in Jamaica, covered the field in the first 300m before showcasing his endurance and strength in the last 100m on his way to a new personal best, national record and world leading 43.74, his first time under 44 seconds.
American Vernon Norwood ran a personal best 44.10 in second while Trinidadian Jereem Richards scaled to new heights in the event with a personal best of his own, 44.18, in third.
Signaling the speed on display in the race, Britain’s Charlie Dobson ran a personal best 44.23 in fourth while Olympic and World champion Kirani James’ season’s best 44.38 was only good enough for fifth.
The women’s 200m saw St. Lucian Commonwealth Games 100m silver medallist Julien Alfred produce a personal best and national record 21.86 for second.
American Olympic bronze medallist Gabby Thomas won in a meet record 21.82 while British 2019 World Champion Dina Asher-Smith was third in a season’s best 22.07.
The women’s 800m saw multiple time World Championship and Olympic finalist Natoya Goule-Toppin run a season’s best 1:56.83 for fourth.
Great Britain swept the top three spots led by World Championship silver medallist Keely Hodgkinson’s personal best, national record and world leading 1:54.61. Jemma Reekie was second in a personal best 1:55.61 while Georgia Bell also ran a personal best 1:56.28 in third.
The 25-year-old Scot clocked a time of 2:02.72 as she finished 0.82 seconds behind Ethiopia’s Tsige Duguma.
Reekie told the BBC: “I knew those girls were going to throw something at me that they were confident with, and they were just better than me today.
“I didn’t want anything other than the win, but first senior medal, I made some mistakes and I’ll learn something from it.
“It’s my first senior medal and I’ve got to take it. I’ve got one now and I definitely want one of those Olympic ones, and it will be a good stepping stone forwards.”
There was also bronze for Great Britain in the women’s 4x400m relay earlier in the evening as the team finished with a haul of four medals.
Laviai Nielsen, twin sister Lina Nielsen, Ama Pipi and Jessie Knight again set a new national record – as they had done in the morning’s heats – with a time of 3:26.36.
They came in behind the Netherlands (3:25.07) and the United States, with Jamaica not finishing after the baton came out of Charokee Young’s hand on the third leg, seemingly via accidental contact from Pipi.
Pipi said: “It was a really messy leg but I just stayed focused on what I needed to do and tried to give it to Jessie in a good position, and I think I did that.”
GB’s other two medals had come in the form of golds on Saturday for Reekie’s fellow Scot Josh Kerr in the men’s 3,000m and Molly Caudery in the women’s pole vault.
British pair Georgia Bell and Revee Walcott-Nolan were fourth and sixth respectively in the women’s 1500m final, and team-mate Cindy Sember was seventh in the women’s 60m hurdles, won in a new world record time of 7.65secs by Devynne Charlton of the Bahamas.
The men’s 1500m final included GB’s Adam Fogg coming 14th.
Also among Sunday evening’s finals was the men’s pole vault title being retained by Sweden’s Olympic champion Armand Duplantis.
The 25-year-old Scot bided her time in second spot before passing Ethiopia’s Habitam Alemu on the final straight to win heat two in commanding fashion in a time of one minute 58.28 seconds.
World number five Reekie progresses to Sunday’s medal race as the fastest qualifier across the two semi-finals and had a warning for her podium rivals.
She told BBC Sport: “(It was) perfect planning – you’d think Jon (Bigg, her coach) knew a bit about this sport by now! (It was) really good.
“I’m in really good shape. Obviously the final’s going to be really tough but I want them to know if they’re coming to win on my track they’re going to have to work hard.
“I think it will be a fast one.”
