Dani Olmo and Pau Victor will be allowed to play for Barcelona after Spain's National Sports Council (CSD) permitted the Catalan giants to provisionally register the duo.
Barcelona signed the two players in pre-season but due to the club being unable to meet LaLiga's wage cap rules, they could only be registered for the first half of the campaign.
On Saturday, the Spanish Football Federation (RFEF) and LaLiga rejected an appeal to register them both for the second half of the season.
"This measure, which is provisional until the final resolution of the appeal lodged by the club and the aforementioned players, suspends the agreement of the monitoring committee of the RFEF-LaLiga coordination agreement... and the cancellation of the sporting licences of the aforementioned players," the RFEF said.
"Likewise, it maintains the validity of those licences until this appeal is finally resolved."
Two separate courts had also rejected Barcelona's requests but the CSD upheld the club's appeal, stating that professional athletes "have the right to a sporting career in accordance with their potential with guarantees and certainty."
"The CSD considers that the non-adoption of this precautionary measure would cause serious economic and sporting damage to the club and, above all, to the players," the CSD continued.
"This could also harm the interests of the Spanish national team, as well as the rest of the national competitions, including LaLiga."
However, the verdict came after Hansi Flick announced the squad for Barcelona's Supercopa de Espana semi-final against Athletic Bilbao later on Wednesday, with neither Olmo nor Victor named among the starters or substitutes.
Olmo – who was signed from RB Leipzig on a six-year deal in August for around €55 million – and Victor were only registered for the first half of the season and their four-month allowance expired on December 31.
The club had initially managed to register Olmo after long-term injuries kept other first-team players out of the squad for the first half of the campaign.