Miami Heat's Bam Adebayo starred for the 16-time champions, registering a team-high 18 points and seven rebounds in their 103-86 triumph at the Pierre Mauroy Stadium.
The USA led from the first quarter and never looked back, with 14 of Adebayo's points coming before half-time as their star-studded bench maintained their advantage.
For South Sudan, Nuni Omot led the way with a game-high 24 points, but with a team boasting 12 NBA All-Stars, four NBA MVPs and six NBA champions, it proved too much for the Bright Stars.
Kevin Durant (14), Anthony Edwards (13), LeBron James (12), Devin Booker (10) and Derrick White all finished in double figures to ensure there was no repeat of their close encounter with their opponents prior to the Games.
South Sudan led the United States by 16 points in their pre-tournament fixture, but narrowly avoided a huge upset in London as they emerged 101-100 victors.
The United States will face winless Puerto Rico in their final group game this Saturday.
Meanwhile, in the other Group C fixture, Nikola Jokic made Olympic history as he helped Serbia ease to their biggest win at the Games following a 107-66 triumph over Puerto Rico.
Jokic finished with 14 points, 15 rebounds, and nine assists, becoming the first player in Olympic history to record at least 10 points, 15 rebounds, and five assists in a single game.
Filip Petrusev also played his part in the victory, with Serbia knowing they must beat South Sudan in their final group game to qualify for the knockout stages.
Charlton won the fifth heat in 8.02, while Anderson finished third in the sixth heat, in 8.10.
The region will be well represented in the semi-finals of the Men’s 60m as well. Mario Burke of Barbados and Jamaica’s Nigel Ellis both ran 6.64, a personal best for Ellis, to finish second and third in the second heat to advance.
Trinidad and Tobago’s Jerod Elcock finished second in heat five with 6.63 to progress, while heat six saw Guyana’s Travis Collins and Rikkoi Brathwaite of the British Virgin Islands run the same time (6.66) to finish as the top two.
The semi-finals and finals of both the Women’s 60m Hurdles and Men’s 60m are scheduled for later on Saturday.
Jamaica's Natoya Goule won her heat in 2:01.65 to advance in the Women’s 800m. The final is scheduled for Sunday.
Dallas Mavericks star Doncic had 31 points, 13 assists and 11 rebounds as Slovenia defeated hosts Lithuania 96-85 in Kaunas on Sunday.
In other qualifying tournament finals, Italy stunned Serbia 102-95, Germany defeated Brazil 75-64 and the Czech Republic downed Greece 97-72.
With those countries headed to Tokyo later this month, the field is now set.
Group A will consist of defending champions the United States, France, Iran and the Czech Republic, with Australia, Nigeria, Germany and Italy in Group B.
In Group C, hosts Japan will contend with Argentina, Spain and Slovenia.
Doncic and Slovenia face a difficult group, as Spain won the bronze medal at Rio 2016 after a close loss to the USA in the semi-finals, but the 22-year-old was ecstatic after qualifying.
"I don't care about the MVP," Doncic said. "We won here. We're going to the Olympics, the first time in our country.
"It's amazing. I think every kid dreams about being in the Olympics. I did too. So, here we are. We fought really, really hard, and I think we deserve to be here."
Italy's defeat of short-handed Serbia, who were missing NBA MVP Nikola Jokic, was the biggest upset of the final qualifying round.
Serbia were beaten finalists against Team USA in the Rio 2016 gold medal game, while Italy are in the Olympics for the first time since taking silver in Athens in 2004.
Williams, who was selected on Jamaica’s team for the 60m, started her season on January 14 at the Purple Tiger meet in Baton Rouge, Louisiana, with times of 7.20 in the preliminaries and 7.18 in the final.
On January 29th, the Olympic 4x100m relay gold medallist ran 7.22 to finish fourth at the Millrose Games in New York. She returned to New York a week later at the New Balance Indoor Grand Prix running 7.09 in the preliminaries, a personal best, and 7.11 in the final.
“The Briana that ran 7.19 in January and 7.09 in February has improved steadily and I think she’s ready to be a factor in Serbia,” Boldon, the four-time Olympic and two-time World Championship medallist, said in an interview with Sportsmax.Tv.
“Is there another 60m PR coming? I believe so. That’s why we are going,” he added.
The World Indoor Championships will be held from Friday, March 18-Sunday, March 20 in Belgrade, Serbia.
Ewa Swoboda (6.99), Marybeth Sant-Price (7.04), Mujinga Kambundji (7.05), and Mikiah Briscoe (7.07) are the only participants that have gone faster than Williams this season.
Charlton capitalized on a bullet start to cross the finish line in 7.81, a Bahamian national indoor record, while Anderson ran 7.85 for second.
Trinidad and Tobago’s Jerod Elcock advanced to the final of the Men’s 60m after finishing second in the first semi-final in 6.63 behind the USA’s Marvin Bracy who eased to 6.51 for the win.
A big clash is expected in the final, later on Saturday, between Italy’s Olympic 100m champion Lamont Marcell Jacobs and 2019 World 100m champion and 2018 World Indoor 60m champion and world record holder Christian Coleman. Jacobs won semi-final two in a world-leading 6.45 while Coleman won the third semi-final in 6.51.
Serbia entered the fourth quarter of the game with a 13-point lead, but a stunning late comeback from the USA, who have won gold in each of the last four Olympics, instead saw them prevail 95-91.
Curry starred for Team USA, scoring 36 points, the second-highest scoring game by an individual in the team's Olympic history, and his two free throws in the dying seconds ensured Serbia were unable to force any more drama.
And Curry explained how the lure of a fifth consecutive Olympic gold medal spurred them to avoid the shock defeat.
"We talked about it the whole way, all summer," he said. "No matter how it looked, we were going to be challenged at some point.
"Outside of the exhibition series we had a pretty smooth road. Serbia are tough. They're a great team.
"Playing them three times, it's always hard to beat a team that many times, so to come back the way we did was amazing.
"We have no quit, and we know how much this means to win a gold medal with this group and the moment we have in front of us.
"Obviously, I hit the big three, but our defence carried us. Defence wins games in any league, especially in FIBA, so it was pretty special."
Serbia at one point held a 17-point lead in the first half, with NBA MVP Nikola Jokic getting 17 points and 11 assists, though they floundered in the closing stages.
While Curry took most of the headlines, LeBron James made his mark too, tying the game at 84-84 with four minutes remaining.
He got 16 points, 12 rebounds and 10 assists to finish with a triple-double, becoming the first player ever to complete the feat twice at the Olympics.
He echoed Curry's sentiments on Serbia's strength, noting how that helped to get the best out of the USA.
"Give a lot of credit to Serbia," James said. "They gave us everything they had.
"It's a great team. They played exceptionally well. They tested us and we needed that."
The USA will face hosts France in the gold-medal match on Saturday.
Serbia gave up a 13-point lead in the fourth quarter against the United States on Thursday as they narrowly missed out on a place in the final, but they responded strongly to ensure they did not leave Paris empty-handed.
Jokic was dominant in that game, and he provided the spark once more on Saturday as he finished with a triple-double, getting 19 points, 12 rebounds and 11 assists.
Vasilije Micic matched Jokic's tally of 19, while Bogan Bogdanovic, the program's all-time leading scorer, added 16.
After a promising start to the Olympics by Germany, they have trailed off in recent matches, and they struggled to match the early pace set by Serbia as they fell 30-21 behind in the first quarter.
Though they matched Serbia evenly in the second quarter, any hopes of getting their hands on their first Olympic basketball medal soon faded in the third, with the 19-point gap proving too much for them to turn over.
Serbia claimed their first medal in the Olympic event since earning silver in Rio 2016.
Adding to a bronze medal in the 400m from the 2014 World Indoor Championships in Sopot, Miller-Uibo got a good lead on the field at the start and used her strength to carry her home in a time of 50.31. Dutch 400m Hurdles Olympic bronze medallist Femke Bol was second in 50.57, while Jamaica’s Stephenie Ann-McPherson was third in a national indoor record 50.79.
Trinidad and Tobago’s Jereem Richards ensured the Caribbean swept the 400m events with a brilliant championship record 45.00 to win gold ahead of the USA’s Trevor Bassitt (45.05) and Sweden’s Carl Bengstrom (45.33).
The 2017 World Championships 200m bronze medallist now has two World Indoor Championships medals after his bronze from the 4x400m in the 2012 edition in Istanbul.
Ukraine’s Maryna Bekh-Romanchuk produced a lifetime best of 14.74m to bump Jamaica’s Kimberly Williams, who jumped a season-best 14.62, down to third.
Williams medal means Jamaica now has two medals from the championships following Stephenie-Ann McPherson’s bronze medal in the 400m on Saturday.
However, it was Rojas, who stole the spotlight when on her final jump, she flew beyond her own world record of 15.43m, to establish a new mark and become the first woman to win three world indoor titles.
She also shattered the previous championship record of 15.36 set by Tatyana Lebedeva in 2004. She has equalled that mark on her penultimate jump of the competition.
Rojas already owned the world-leading mark indoors with 15.41m she set in Spain on March 2.
Team USA – who have won gold at the last four editions of the Olympics – looked set to miss out on Saturday's final when Serbia entered the fourth quarter with a 13-point lead.
NBA MVP Nikola Jokic had 17 points and 11 assists for Serbia, who led by as many as 17 points at one stage in the first half.
Bogdan Bogdanovic had 20 points for Serbia while Aleksa Avramovic had 15, but Serbia were unable to hold on and avenge their 110-84 loss to the USA in the group stage.
Golden State Warriors star Curry carried the USA's offense for most of the game, though it was LeBron James' driving layup that tied the contest at 84-84 with under four minutes remaining.
The USA struggled defensively for long periods but finally began to put real pressure on Serbia late in the fourth quarter, and a missed 3-pointer from Ognjen Dobric with Serbia 86-84 up proved decisive.
Curry put the USA ahead with a 3-point jumper on a James assist with two minutes and 24 seconds left, then layups from both players stretched their lead as Serbia wilted.
Twice Serbia drew within two points in the final minute, only for two Curry free throws to seal the USA's victory with eight seconds on the clock.
They will face hosts France – led by NBA Rookie of the Year Victor Wembanyama – in Saturday's gold medal match, after Serbia face Germany for bronze.
James added 12 rebounds and 10 assists to his 16 points to finish with a triple-double, while USA team-mate Joel Embiid brought up 19 points.
Durant did not play any of the pre-Olympic warm-up games due to a calf injury and was only cleared to play for the USA an hour before their opener started.
However, he made a huge impact in just 17 minutes, nailing his first eight shots, including five 3-pointers, as he scored 23 points.
After being touch-and-go for the tournament, with coach Steve Kerr reluctant to rush him back too early, Davis says the team knew he would be ready when called upon.
"We expect nothing less," he said. "We know what he can do when the ball is in his hand, and he got hot. It was fun to watch his first time, second time, 12th.
"To see and be a fan and actually get a chance to cheer for him instead of rooting against [him] with his scoring ability – man, it was fun to watch."
Durant was not the only one in fine form, as LeBron James marked his Olympic comeback with a standout performance, getting 21 points, eight rebounds and nine assists as he provided a spark.
James and Durant also became the first USA team-mates to both score over 20 points in an Olympic opener.
The 39-year-old, playing his first contest at the Games since 2012, when he won his second consecutive gold medal, was full of praise for Durant and the team as the USA chase a sixth title in a row.
"I mean [Durant] is just an out-of-this-world talent," he said after the game. "That's the best game we played so far.
"[I'll do] Whatever it takes [to win]. It's going to be somebody different every day. And we have that type of firepower."
Next up for Team USA in Group C is South Sudan, who made history in their Olympic debut earlier on Sunday.
Their basketball federation was only formed in 2011, and they beat Puerto Rico 90-79 for their first win at the Games.
