Jabeur reached the finals of Wimbledon and the US Open last season but was unable to build on that strong finish to 2022 at the start of this year.
The Tunisian exited the Australian Open in the second round, having complained of a knee injury.
Jabeur said after her defeat to Marketa Vondrousova she would need "time to recover and get healthier", and she has not been seen on the WTA Tour since.
The Qatar Open was due to see Jabeur's return next week, but an Instagram post has now confirmed she will miss both that tournament and the subsequent Dubai Tennis Championships.
"In order to take care of my health situation, my medical team have decided that I need to get a minor surgery in order to be back on the courts and perform well," she wrote.
"I will have to retire from Doha and Dubai, and this is breaking my heart.
"I would like to say sorry to all the fans out there in the Middle East that waited for this reunion.
"I promise I will come back to you stronger and healthy."
Jabeur will continue to play in the doubles, where she will pair with American legend Serena Williams, who has not played a competitive match since a first-round defeat at Wimbledon last year.
The news of the Tunisian's withdrawal from the singles tournament comes on the same day that she was elevated to world number three, after her victory at the Berlin Open on Sunday.
She beat Belinda Bencic in the final after her Swiss opponent retired with an ankle injury.
"I feel like I need to be smart about this decision," Jabeur told reporters about her choice not to play singles at Eastbourne.
Jabeur and Williams will face off against the unseeded duo Marie Bouzkova and Sara Sorribes Tormo in the first round on Tuesday.
Jabeur, a finalist at both Wimbledon and the US Open this past season, received a bye through to the second round thanks to her status as the second seed, and she had to save three break points in the opening game of the match against Tsurenko.
After repelling the early push, Jabeur rattled off the first five games to take a commanding lead, and she secured another break to begin the second frame.
Meanwhile, with half of the field still completing their first-round matchups, 12th seed Paula Badosa proved too strong for Egypt's Mayar Sherif in a comfortable 6-3 6-1 victory.
Spain's Badosa will play Canada's Leylah Fernandez in the second round after the promising 20-year-old took just 72 minutes to dispatch Russia's Evgeniya Rodina 6-3 6-2.
Rodina was the only loss from the four Russians in action on Tuesday, with Anna Kalinskaya defeating Alize Cornet 7-6 (7-4) 4-6 6-2, 19-year-old Diana Shnaider eliminating Alycia Parks 6-4 6-3, and Varvara Gracheva prevailing 7-6 (7-3) 6-2 against Anna-Lena Friedsam.
Bernarda Pera advanced past Clare Liu 6-4 6-7 (1-7) 6-3 in one of the all-American showdowns, while ninth seed Madison Keys beat Emma Navarro 6-4 6-3 and Shelby Rogers took out Danielle Collins 6-7 (2-7) 6-4 6-1 in the others.
Sofia Kenin, the 2020 Australian Open champion, handled the challenge of Aliaksandra Sasnovich 6-1 6-7 (5-7) 6-1, and two-time Australian Open champion Victoria Azarenka came back to defeat Sloane Stephens 3-6 6-3 6-2.
Osaka fell at the first hurdle to Angelique Kerber at Roland-Garros last month, but was back to her best as she emerged a 6-3 6-1 victor in Toronto.
The four-time grand slam champion wasted no time in stamping her authority on proceedings, earning a two break points to take a 3-0 lead early on.
And that would prove decisive, with the world number 95 seeing out the rest of her service games, closing out the first set with a love game.
Jabeur would have no response to Osaka's stunning display, with the Japanese finding another pivotal break before winning the remaining three games to confirm the win.
Osaka will face Elise Mertens in the round of 32 after the Belgian beat Katie Volynets in straight sets earlier in the day.
Data Debrief: Osaka over Olympics disappointment
Having suffered disappointment in Paris, Osaka responded in emphatic style upon her return to the court and will want to continue that momentum moving forward.
Osaka converted all three of the break points she had, winning all eight of her service games.
It proved to be a difficult day at the office for Jabeur, who uncharacteristcally served seven double faults throughout the contest compared to Osaka's four.
The four-time grand slam champion defeated world number 16 Jabeur 6-3 6-1 in her first-round match in Toronto.
Osaka’s victory follows her early exit in the Olympics where she lost in straight sets to Germany’s Angelique Kerber last month at Roland-Garros.
Away from the clay courts, the Japanese player felt the surface and her opponent brought out the best in her in Canada.
“Everyone knows I really love hard court,” said Osaka. “So I don’t know, I wasn’t really thinking too much.
"It felt very instinctual and honestly when I play the best players like Ons I tend to play better."
Now, Osaka believes she is beginning to build momentum again after her hiatus.
“I think if you’ve been following my journey a little, I go through mountains and hills. But now I’m feeling quite confident in myself as a person and a player and I think that showed a little,” she explained.
“Hopefully I can continue to play a lot more matches, but I think I just really love playing tennis and it’s been an honour to play such a great match in front of everyone.”
It is the first time since the 2012 Stuttgart Open that all four top seeds reached the final four in a WTA 500 event.
Pegula, the top overall seed and the only remaining American, used her commanding serving game to overwhelm 12th seed Paula Badosa 6-3 7-6 (8-6).
She ended up winning 80 per cent of her accurate first serves – compared to 57 per cent for Badosa – and it resulted in the Spaniard producing just one break point opportunity in the match, which she could not take.
Pegula will meet fourth seed and Olympic gold medallist Belinda Bencic next after her relatively comfortable 6-3 6-3 triumph against Russia's Ekaterina Alexandrova.
Switzerland's Bencic needed just 80 minutes to get the job done, creating 12 break point chances compared to Alexandrova's two, while winning the first three games of each set.
After coming into the tournament without consecutive wins since early January, reigning Wimbledon and US Open finalist Ons Jabeur looked back to her best in a dominant 6-0 4-1 (retired) drubbing of Anna Kalinskaya.
Jabeur is yet to lose a set in Charleston, and she will try to keep that the case when she faces Daria Kasatkina in her semi-final.
Kasatkina earned her spot in the final four with Friday's only three-setter, emerging victorious 6-7 (5-7) 6-4 6-2 against Madison Keys in just over two and a half hours.
Pegula, the top-ranked American and the tournament's overall top seed, may have thought it was going to be smooth sailing after collecting a break-to-love in the match's opening game, but it was just the beginning of a two-hour-and-32-minute battle.
Begu played terrific tennis against a world-class opponent, winning 47 per cent of the match's total points, but she shot herself in the foot with eight double faults while Pegula had just one.
With the victory, Pegula booked her place in the quarter-final against Spain's Paula Badosa after her stylish 6-1 6-3 result over Russian 19-year-old Diana Shnaider.
Badosa now has a 4-2 record since the beginning of March, but both of those losses came to the in-form Elena Rybakina in eliminations from both the Indian Wells Open and the Miami Open.
Meanwhile, reigning Wimbledon and US Open finalist Ons Jabeur needed just 82 minutes to dispatch Caroline Dolehide 6-3 7-5, and combined with her opening victory against Lesia Tsurenko it is Jabeur's first consecutive wins since the Adelaide International in early January.
Jabeur will play Anna Kalinskaya in her quarter-final after the Russian upset two-time Australian Open champion Victoria Azarenka 6-4 7-6 (7-5).
It was a strong day for the Russians, as Ekaterina Alexandrova got the better of Julia Grabher 6-4 6-2, and world number eight Daria Kasatkina knocked out Bernarda Pera 6-3 7-6 (7-3).
Olympic gold medalist Belinda Bencic won the longest match of the day in a two-hour-and-45-minute war of attrition against Shelby Rogers 4-6 7-5 6-2, but the Americans had something to cheer for in the late window as Madison Keys advanced past Magda Linette 6-2 3-6 6-1.
Pegula led Gauff 7-5 6-6 (3-1) when rain in the German capital halted play on Saturday, with a later announcement confirming the match will not resume until Sunday.
Both players had already taken to the court once on Saturday, as Gauff's last-eight opponent Ons Jabeur retired due to illness after losing a 68-minute opening set 7-6 (11-9).
Pegula, meanwhile, wrapped up a two-day quarter-final victory over Katerina Siniakova by a 7-6 (7-2) 3-6 6-3 scoreline.
The winner of the pair's match will also have to play twice on Sunday, with Kalinskaya lying in wait in the final after she beat former world number one Victoria Azarenka 6-1 6-7 (3-7) 6-1.
Data Debrief: Kalinskaya primed for an upset?
Whoever emerges victorious on Sunday morning, Kalinskaya will face a top-five player, with Gauff currently second in the world rankings and Pegula fifth.
Kalinskaya has already recorded three top-five victories this year. She lost her only previous meeting with Pegula in Washington D.C. in 2019 but won her first clash with Gauff in Dubai earlier this year.
The world number 12 was trailing 6-2 2-1 when calling time on the match against Andreescu in a highly anticipated showdown between two of the past three US Open winners.
Raducanu initially left the court for treatment ahead of the seventh game of the opening set for a medical timeout and completed just four more games.
Speaking after her withdrawal, coming two weeks before the French Open, Raducanu explained she did not want to aggravate an injury sustained at last week's Madrid Open.
"I thought maybe taking one, two days off, it would go away because a lot of the other small niggles I've had, they've kind of gone away after taking, like, two days off," she said.
"Then I got here and I was training, but it just didn't seem to get better. I was training with some limitations. I wasn't moving really.
"I was just playing where I knew where the ball was coming, just staying in one corner. I think I must have underestimated the unpredictability of competition in a match.
"The last few weeks have been really positive. I've learned a lot about myself and my game has definitely improved on this surface but I need to make sure my back is fully right.
"I need to just keep on it. I don't want to play my next match with a feeling of limitation because I think that I learned my lesson from this week, when to push, when not to push."
Ons Jabeur joined Andreescu in the last 32 with a 6-0 7-6 (7-1) victory over Sorana Cirstea to keep her impressive run of form going.
The Tunisian won her first WTA 1000 title in Madrid last week and has now won seven successive main draw matches for the first time in her career
Elsewhere in Tuesday's action, American qualifier Lauren Davis pulled off a shock 6-2 6-3 win against number 11 seed Jelena Ostapenko.
Fellow Americans Jessica Pegula, Madison Brengle and Amanda Anisimova also advanced, beating Liudmila Samsonova, Marta Kostyuk and Tereza Martincova respectively.
Anisimova, who is now the player with the most wins in three sets so far in 2022 with nine, will face Belinda Bencic for a place in the last 16.
Raducanu dished out a bagel to qualifier Viktoria Kuzmova in the first set of the round of 16 match in Auckland on Thursday, but the 2021 US Open champion was unable to continue after losing the second set 7-5.
The 20-year-old Brit ended a disappointing 2022 season early due to a wrist injury and suffered another blow ahead of the first major of this year, leaving the court in tears.
Gauff will be eyeing her maiden grand slam title in 2023 and she impressively dispatched fellow American Kenin 6-4 6-4 in the second round in the capital of New Zealand.
Top seed Gauff and her compatriot were forced to play indoors due to the rain and it was the 18-year-old who prevailed, breaking three times and saving six of the break points she faced.
The teenager will face Zhu Lin of China for a place in the semi-finals after she beat Venus Williams 3-6 6-2 7-5.
Leylah Fernandez inflicted a crushing 6-0 6-1 defeat on Julia Grabher after losing only two games in her first-round win over Brenda Fruhvirtova and the Canadian will now face Ysaline Bonaventure, who defeated Rebecca Marino in three sets.
Top seed Ons Jabeur made a winning start to the season at the Adelaide International, seeing off Sorana Cirstea 7-6 (7-3) 6-1 to set up a meeting with Marta Kostyuk
Irina-Camelia Begu claimed the scalp of Jelena Ostapenko with a 6-3 6-0 upset of the seventh seed, while Victoria Azarenka was a 6-2 7-5 winner against Zheng Qinwen and teenager Linda Noskova got past Claire Liu in straight sets.
Playing in her first tournament since retiring from the opening round of Wimbledon injured last year, Williams acknowledged she was particularly reliant on Jabeur during their 6-2 6-4 success over Shuko Aoyama and Chan Hao-ching.
The 23-time grand slam singles champion opted to only play doubles on the south coast as she gears up for a return to the All England Club.
"I think we played together much better," said Williams. "Although I thought we played really good together [against Maria Bouzkova and Sara Sorribes Tormo on Tuesday] too.
"But Ons really held me up. She was really playing so good. I was looking at her and was like, 'Wow, this is great.' It's good."
Williams and Jabeur will take on Aleksandra Krunic and Magda Linette for a place in the final.
Jabeur playfully asked the 40-year-old if she would like to play together at the US Open, to which the former world number one responded: "Sign me up!"
The Tunisian added: "I'm getting used to this. It was really great to play here and I didn't know we are in the semi-finals. It's super fast, but it's great for us."
Organisers said Tunisian world number three singles star Jabeur was troubled by a right knee injury, forcing her brief alliance with Williams to come to an end.
They had won through two rounds in increasingly impressive style, with Williams making her return to the WTA Tour, having not played competitive tennis since injuring an ankle at Wimbledon last year.
The 40-year-old Williams was clearly enjoying the partnership with Jabeur, who in May became the first African or Arab woman to win a WTA 1000 singles title when she triumphed at the Madrid Open.
They had named themselves 'Onsrena' and were due to face Aleksandra Krunic and Magda Linette in the semi-finals, before news emerged on Thursday of their withdrawal.
No details of the seriousness of Jabeur's injury were disclosed, and it may prove a precautionary withdrawal given Wimbledon is so close, with the latter starting on Monday.
Jabeur won a title on grass in Berlin last week, showing she could pose a threat to top seed Iga Swiatek, providing she is not hampered by injury.
Williams, a 23-time grand slam singles champion, will also be competing at Wimbledon after being handed a wildcard entry into the tournament where she has won seven singles titles.
Williams, playing for the first time since declaring on Tuesday her intention to retire after this month's US Open, was no match for Tokyo Olympics gold medalist Bencic.
The 23-time major winner was unable to claim back-to-back WTA singles wins for the first time since last year's French Open, having defeated Nuria Parrizas-Diaz for her first victory in 430 days on Monday.
Bencic triumphed in one hour and 17 minutes, winning 84.2 per cent of first-serve points and converted five of eight break points throughout the match.
Williams' power was on show with 13 winners, but Bencic was physically more capable and decisively managed 25 winners with only 13 unforced errors.
Elsewhere, 2019 US Open winner and local hope Bianca Andreescu edged Alize Cornet in a see-sawing clash in the evening, winning 6-3 4-6 6-3 in two hours and 26 minutes.
Fourth seed Paula Badosa and fifth seed Ons Jabeur, who was last month's Wimbledon runner-up, were forced to retire due to injury.
Spanish 24-year-old Badosa withdrew against Yulia Putintseva 7-5 1-0 due to muscle cramping, while Zheng Qinwen had a walkover against Jabeur 6-1 2-1 due to abdominal pain.
Top seed Iga Swiatek brushed aside Ajla Tomljanovic 6-1 6-2 in 64 minutes. The win means Swiatek is the first player to win 15 WTA 1000 matches in straight sets in a row since 2009.
Canadian 13th seed Leylah Fernandez also bowed out, going down 7-6 (7-4) 6-1 to Brazil's Beatriz Haddad Maia, who will face Swiatek in the third round.
Second seed Anett Kontaveit lost 6-4 6-4 to Jil Teichmann in one hour and 27 minutes. Teichmann will next face Simona Halep who won in 71 minutes against Zhang Shuai 6-4 6-2.
Sixth seed Aryna Sabalenka got past Sara Sorribes Tormo 6-4 6-3 to set up a third-round meeting with Coco Gauff after she defeated Wimbledon champion Elena Rybakina 6-4 6-7 (8-10) 7-6 (7-3) in an epic that lasted two hours and 49 minutes.
Jessica Pegula won 6-2 7-5 over American qualifier Asia Muhammad to progress through to face Camila Giorgi after she knocked off Elise Mertens 7-3 7-5.
Third seed Maria Sakkari triumphed in three sets 6-2 4-6 6-2 over Sloane Stephens and will face Karolina Pliskova next after the Czech beat Amanda Anisimova 6-1 6-1.
Eighth seed Garbine Muguruza won 6-4 6-4 against Kaia Kanepi and Alison Riske toppled 16th seed Jelena Ostapenko 7-6 (7-2) 0-6 7-5.
Swiatek made a disastrous start, going 4-0 down, and though she broke back once, was unable to find the second as Pliskova took the opening frame 6-4.
The determined Pole stepped up a level though to take the second 6-1, before breaking early in the third.
It was still an improvement for the Czech player, who lost 6-0 6-0 to Swiatek in their only previous meeting in Rome in 2021.
Pliskova kept her opponent honest on her own serve, but Swiatek had too much for the world number 17 as she clinched the decider 6-2.
Aryna Sabalenka also showcased her powers of recovery as she beat Paula Badosa despite going a set down.
The second seed won 4-6 6-4 6-4 in an even contest that saw nine breaks of serve, before the Belarusian eventually got over the line.
Sabalenka is now the player with the joint-most comeback wins over the last two seasons (13) level with Caroline Garcia, though the French star was actually the victim of a comeback herself on Friday.
Garcia won the first set against Anastasia Potapova, before the Russian came back to take it 4-6 6-3 6-3 to set up a semi against Sabalenka.
There was no need for such drama from Ons Jabeur, who eased to a 6-3 6-0 victory against Beatriz Haddad Maia in just 68 minutes to confirm a clash with Swiatek.
The top seed, who made it to the final last year, dropped a set for the first time in this tournament before recovering to win 4-6 6-0 6-2 in a match that lasted just short of two-and-a-half hours.
These two players met in the French Open semi-finals last year and 11th seed Haddad Maia was eyeing an upset when she fought back from 4-1 down to claim the opener.
But Swiatek dropped serve just once in the next two sets as she assumed full control and swept to victory, powered by winning eight straight games from the start of the second set.
Data Debrief: Swiatek statistics up there with the best
Up next for Swiatek will be a last-four clash against either Ons Jabeur or Madison Keys, who play later on Tuesday. That will be her 15th WTA 1000 semi-final appearance since 2020, with no other player having made it to double figures (Aryna Sabalenka and Maria Sakkari are the next best on eight each).
Beating Haddad Maia also means Swiatek has equalled Serena Williams and Maria Sharapova as the fastest player to reach 25 WTA 1000 wins on clay, doing so in just 29 matches.
And Swiatek's emphatic second set saw her tie Coco Gauff for the most number of 6-0 sets won so far this year. They both have seven, just ahead of Sabalenka (five).
Defending champion Sabalenka plays Mirra Andreeva in the last eight on Wednesday after battling past Danielle Collins in three sets. All three of her wins so far have been in deciding sets.
The other last-eight clash is between Elena Rybakina and Yulia Putintseva.
Swiatek, who became the first female player to win 25 consecutive matches since Serena Williams in 2015, will face Bianca Andreescu in the last eight after she eased past Petra Martic 6-4 6-4.
"I didn't start well, and everybody could see that," Swiatek told reporters. "I'm really happy with the way I reacted and how I improved in the first set.
"Also how different the second set looked to the first one because I could really reset and really change the way I played. That's the most positive thing for me."
There was a shock in the final game of the day, though, as Daria Kasatkina dumped out number two seed Paula Badosa 6-4 6-4.
Third seed Aryna Sabalenka sealed a third straight win over Jessica Pegula, easing to a 6-1 6-4 victory, while fourth seed Maria Sakkari defeated Coco Gauff 6-4 7-5.
Seventh seed Danielle Collins was knocked out by unseeded compatriot Amanda Anisimova, the 20-year-old cruising past the Australian Open finalist 6-2 6-2.
There were also victories for Jil Teichmann, who overcame Elena Rybakina 6-7 (3-7) 6-3 7-5, and ninth seed Ons Jabeur, who beat Yulia Putintseva 6-3 6-2.
The world number one faced Ons Jabeur – who had been on an 11-match winning run – in Sunday's final in Rome and simply had too much for the Tunisian.
Swiatek won 6-2 6-2, with the 20-year-old Pole getting an early break to establish a 3-0 lead that put her in control of the first set.
As it happened, last week's Madrid Open champion Jabeur never managed to wrest control back from her opponent – her only break of the match came while 4-1 down in the second, with Swiatek digging deep to save four break points in her next service game.
Swiatek then saw things out to defend her Rome title and add to her impressive recent streak.
Additionally, Swiatek has emerged victorious from every WTA 1000 event she has entered this season, having previously won in Doha, Indian Wells and Miami.
But she was quick to salute Jabeur's efforts after another commendable showing.
Swiatek said: "I want to congratulate Ons because she had such a good run on the clay court.
"You have shown fight, spirit, so much variety that it's really nice to have you on tour. Your tennis is different and your tennis is really interesting for women's tennis I think."
As for her own performances, Swiatek added: "It wasn't easy for the whole week to play every day, but the crowd gave me so much energy.
"It was so nice to play here, be in Rome, and I agree with Ons in terms of good pasta! And today I'll celebrate with a lot of Tiramisu – no regrets!"
Swiatek now heads to Roland Garros – the scene of her first and only previous grand slam success in 2020 – as the firm favourite.
The 21-year-old Pole, who won last year's Indian Wells, had match point at 6-0 5-0, before Liu rallied back on serve to avoid the ignominy of a double-bagel defeat, with Swiatek eventually winning 6-0 6-1.
Swiatek was in a dominant mood, winning 57 of 83 points for the match and converting five of nine break points generated. She dropped only 11 points in seven service games.
The three-time grand slam champion is 13-3 on the season, with all 13 wins coming in straight sets. In five of those matches, she has dropped just one game.
Former US Open winner Emma Raducanu moved into the third round with a 7-6 (7-3) 6-2 win over 20th seed and 2023 Australian Open semi-finalist Magda Linette.
Raducanu's win meant she has put together back-to-back victories for the first time since September, setting up a clash with 13th-seed Beatriz Haddad Maia after she beat Katerina Siniakova 5-7 7-6 (7-4) 6-3.
Fourth seed Ons Jabeur fought back from a set down to prevail over Magdalena Frech 4-6 6-4 6-1 in one hour and 44 minutes.
Former US Open champion Bianca Andreescu also overcame an early deficit to win 4-6 6-4 6-3 over Peyton Stearns.
Reigning Wimbledon champion Elena Rybakina got the edge in a tight two-set clash with 2020 Australian Open winner Sofia Kenin, triumphing 7-6 (8-6) 7-6 (7-5) in two hours and 10 minutes. Rybakina fought back from 4-1 down in the second frame.
Fifth seed Caroline Garcia overcame a wobble to win 6-1 6-7 (4-7) 6-4 over Hungary's Dalma Galfi, with 30th seed Leylah Fernandez her next opponent after beating Emma Navarro 6-2 6-4.
Two-time major winner and three-time US Open finalist Victoria Azarenka was the big casualty from the day's play, losing 7-6 (7-1) 6-3 to 2021 Australian Open semi-finalist Karolina Muchova.
Top seed Switaek, seeking a fifth consecutive title, breezed past Elena-Gabriela Ruse 6-3 6-0 as she became the first female player to win 24 consecutive matches since Serena Williams in 2015.
Badosa followed suit by dispatching Aliaksandra Sasnovich 6-2 6-1 in just an hour and 17 minutes, teeing up a third-round clash with Daria Kasatkina, who beat Leylah Fernandez 4-6 6-3 7-6 (7-2).
Aryna Sabalenka, the third favourite in Italy, also made light work of Zhang Shuai to triumph 6-2 6-0, while fourth seed Maria Sakkari cruised past Madrid Open semi-finalist Ekaterina Alexandrova 6-3 6-2.
Sakkari will next face a Rome rematch with Coco Gauff, who defeated compatriot Madison Brengle 6-2 6-4. Teenager Gauff's sole win against Sakkari came at this tournament in the second round last year.
Yulia Putintseva came from behind to beat Garbine Muguruza 3-7 7-6 (7-4) 6-1, and will next meet Ons Jabeur, who made it eight successive main-draw wins by defeating Ajla Tomljanovic 7-5 6-2.
Seventh seed Danielle Collins also battled past former world number one Simona Halep 7-6 (7-1) 6-3.
There was no such joy for fifth seed Anett Kontaveit as she crashed out to Petra Martic in straight sets, while Jil Teichmann upset former Rome champion Karolina Pliskova in a three-set thriller.
Belinda Bencic, who was ranked as 12th seed, was another early casualty as she fell to a 7-6 (7-4) 6-1 defeat against American Amanda Anisimova.
Meanwhile, Jessica Pegula secured her third-round spot after Anhelina Kalinina withdrew due to injury, with Victoria Azarenka earning a straightforward 6-2 6-4 victory over Camila Osorio to get a shot at favourite Swiatek in the next round.
World number one Swiatek was 3-0 up against Jabeur when her tearful opponent called a medical timeout and ultimately ended the contest early.
It sets up a mouthwatering showdown between top seed Swiatek and second seed Sabalenka, who defeated Anastasia Potapova in straight sets earlier on Saturday.
Jabeur, who struggled to shake off the injury sustained in the first game, said: "The third point, I don't know what happened.
"I was really excited to play Iga today – it's always a fun match between us. I wish her the best luck for the final. I'm sorry guys, I really tried to run, but Iga never makes it easy."
Sabalenka, a beaten finalist in the past two years, held her serve in the opening set against Potapova and twice broke her unseeded opponent to take the lead.
The Belarusian raced 4-0 ahead in the second set and saw out a comfortable 6-1 6-2 victory in a little under an hour to reach her fourth final of the year.
"I think I was really focused, and I think from the very first point I was playing really aggressive," Sabalenka said in her on-court interview. "I didn't give [Potapova] much time.
"I think that's why the match finished like that quick. But anyway, she's a great player, and I think in the future she's going to be a top player."
Swiatek defeated Sabalenka in last year's Stuttgart final 6-2 6-2 and is aiming to become the first player to defend the title since Angelique Kerber in 2016.
