Higuain converted an 81st-minute free-kick and it proved to be the winner as Inter Miami defeated New York Red Bulls 2-1 on Wednesday.
The former Argentina international, who joined David Beckham's Inter Miami from Serie A champions Juventus, missed a penalty on debut last month.
In his third appearance for Inter Miami, Higuain earned a free-kick with nine minutes remaining and stepped up to beat Red Bulls goalkeeper David Jensen with a powerful shot into the top corner.
Higuain's first MLS goal came via a direct free-kick after not scoring a set-piece in 428 matches during his time in the top five European leagues, per Opta.
"That's my role," Higuain said in a post-match news conference. "I've been playing in the best leagues in the world and that's my main function, to score."
The Red Bulls had led through Omir Fernandez's 53rd-minute opener, before Matias Pellegrini equalised two minutes later in New Jersey.
Inter Miami are 12th in the 14-team Eastern Conference with 14 points from 16 games, while the Red Bulls are six points better off in seventh.
The visiting Timbers defeated the struggling Galaxy in a wild nine-goal showdown in Carson, California.
Portland led 2-1 at half-time before scoring another four goals in a thrilling encounter at Dignity Health Sports Park.
Felipe Mora and Jeremy Ebobisse each finished with braces as the Timbers scored a joint-club record six goals for the second time in four matches, having not scored more than five times in any of their first 337 MLS matches, via Opta.
The Galaxy have lost five matches in a row to sit bottom of the Western Conference, while the Timbers are second and three points adrift of leaders Seattle Sounders – who edged Real Salt Lake 2-1.
Elsewhere, New York City eased past DC United 4-1, Eastern Conference leaders Toronto downed New England Revolution 1-0 and Philadelphia Union were too good for FC Cincinnati 3-0.
Dallas lost 2-0 to Houston Dynamo, Sporting Kansas City were 1-0 winners over Chicago Fire, San Jose Earthquakes beat nine-man Vancouver Whitecaps 3-0, Montreal Impact topped Columbus Crew 2-1, while Atlanta United and Orlando City played out a goalless draw.
The Colorado Rapids-Los Angeles FC clash was postponed after one new confirmed case of coronavirus among the Rapids staff.
Galaxy stretched their winning streak to four games with a 3-0 victory in the derby clash at Dignity Health Sports Park on Sunday.
Cristian Pavon opened the scoring in the 51st minute, lobbing Pablo Sisniega after the LAFC goalkeeper was left stranded having come to attempt to collect an Emiliano Insua pass.
Just two minutes later and LAFC were dealt another blow, Mark-Anthony Kaye sent off for a rash challenge on Julian Araujo.
Galaxy capitalised on their numerical advantage as Sebastian Lletget tucked away a smart Pavon set-piece in the 73rd minute.
And 10 minutes later Lletget sealed the win, his overhead kick from Rolf Feltscher's cross finding the corner.
Galaxy jumped into fourth in the Western Conference after a second straight El Trafico win.
Columbus are five points clear atop the Eastern Conference after a 3-0 win over FC Cincinnati.
The Crew needed until the second half to break the game open at MAPFRE Stadium in Ohio.
Pedro Santos opened the scoring in the 52nd minute before Gyasi Zardes' brace.
Zardes got on the end of a Santos cross before later scrambling in to see Columbus to a seventh win in 10 MLS games this season.
Philadelphia Union are second to Columbus and recorded a 3-0 win over New York Red Bulls.
Minnesota United scored four second-half goals in a 4-0 win over Real Salt Lake, New England Revolution edged Chicago Fire 2-1 and Portland Timbers recorded a 2-1 victory against Seattle Sounders.
Inter Miami's struggles continued with a 0-0 draw against Nashville as former Juventus and Paris Saint-Germain midfielder Blaise Matuidi made his debut, while DC United and New York City also drew 0-0.
Coming off a defeat to Los Angeles FC, Inter Miami went down to DC United 2-1 on Saturday.
Rodolfo Pizarro scored the first MLS goal in the club's history, sweeping in a Lewis Morgan cross to give them the lead in just the second minute.
However, the game turned early in the second half when, after Morgan thought he had doubled his side's lead, the visitors had the goal ruled out and Roman Torres sent off for a handball.
DC equalised through Yamil Asad's penalty in the 59th minute before finding what proved to be the winner just after the hour-mark.
Edison Flores hit the post with a free-kick and Frederic Brillant reacted quickest to fire in the rebound and lift DC to their first win of the season.
LA Galaxy are without a win through two games after suffering a 1-0 loss at home to the Vancouver Whitecaps.
Tosaint Ricketts' smart side-footed finish in the 74th minute was enough for the Whitecaps.
Joe Corona was then sent off for Galaxy, who were beaten despite David Bingham later saving a Lucas Cavallini penalty.
Without the injured Josef Martinez, Atlanta United made it two wins from two by beating Cincinnati 2-1.
There are three teams with perfect records in the Western Conference in Sporting Kansas City, Minnesota United and the Colorado Rapids.
Sporting KC thrashed Houston Dynamo 4-0, Ike Opara netted a brace in Minnesota's 5-2 win over the San Jose Earthquakes and Colorado edged Orlando City 2-1 thanks to Drew Moor's late winner.
New England Revolution and Chicago Fire drew 1-1, Real Salt Lake came from behind to hold the New York Red Bulls to a 1-1 draw and Maximiliano Urruti's brace helped Montreal Impact draw 2-2 with Dallas.
Ifunanyachi Achara's goal on his MLS debut saw Toronto overcome New York City 1-0, while the Seattle Sounders drew 1-1 against Columbus Crew.
City defender Alexander Callens netted the game's only goal in the 29th minute, while goalkeeper Sean Johnson was outstanding for the visitors.
Callens powered in the winner with a bouncing header from Santiago Rodriguez's corner.
Minnesota almost equalised in the 90th minute but were thwarted by a spectacular double save by Johnson from Emanuel Reynoso and Robin Lod efforts.
City moved ahead of Philadelphia Union in the east, after they could only manage a 1-1 draw away to under-achieving New England Revolution.
The Revs, who won last season's Supporters' Shield, have rallied after a poor start to their campaign, going unbeaten in their past five, but have only collected 16 points from 13 games.
Gustavo Bou had put New England ahead from the penalty spot in the 75th minute, but Union levelled two minutes later after Omar Gonzalez's inexplicable error allowed Mikael Uhre to score.
Montreal, who are third in the east, got back on the winners' list with a hard-fought 4-3 victory over Cincinnati with a double from Rommel Quioto.
Luquinhas netted a brace as New York Red Bulls stayed in touch with the Eastern Conference's top three with a 4-1 home victory over struggling DC United.
In the Western Conference, LAFC remains four points clear at the top after Brian Rodriguez's 47th-minute winner clinched a 3-2 triumph over San Jose Earthquakes.
LAFC had raced to a 2-0 lead inside 13 minutes but San Jose equalised after two goals from in-form Jeremy Ebobisse, who has scored an equal-league high nine times this term.
Rodriguez, who got the assist for Ryan Hollingshead's 13th minute goal, scored when a loose ball arrived at his feet unmarked at the back post, firing past Earthquakes goalkeeper JT Marcinkowski.
Nashville ended the Colorado Rapids' remarkable 23-game unbeaten run at home with a 3-1 victory.
Charles Sapong netted in the eighth minute, before Hany Mukhtar's quickfire double had Nashville 3-0 up inside 18 minutes. The win moves Nashville up to fifth in the west.
Dallas came from behind to win 3-1 at Orlando City, Real Salt Lake won their third straight with a 3-0 victory over Houston Dynamo and Vancouver Whitecaps moved off bottom spot with a 1-0 triumph at Sporting KC.
Inter Miami extended their unbeaten run to four games with a 2-1 home win over Portland Timbers, Columbus Crew triumphed 2-1 over 10-man Atlanta United and Toronto won 3-2 over Chicago Fire after two late Pozuelo goals.
LAFC led 2-1 and then 3-2 heading into the 86th minute but Cabral's late heroics earned a share of the points for the Galaxy in an MLS thriller on Saturday.
Signed from Bundesliga outfit Eintracht Frankfurt this month, Serbia international Joveljic – experiencing his first El Trafico derby – broke the deadlock in the 20th minute.
LAFC – who had lost a club record four consecutive matches as part of their record seven-match winless run heading into the fixture – were back on level terms courtesy of Cristian Arango's penalty on the stroke of half-time.
A moment of brilliance from Brian Rodriguez in the 58th minute gave LAFC a 2-1 lead, his stunning individual run evaded several Galaxy defenders before lashing home.
LAFC and the Galaxy traded goals as Jovejlic struck again in the 64th minute before Rodriguez restored the former's lead just two minutes later.
However, there was to be late drama – Cabral pounced on Araujo's cross and scored in front of a stunned home crowd.
The Galaxy are fourth in the Western Conference, six points behind leaders Seattle Sounders and 12 points clear of ninth-placed LAFC.
Elsewhere, Eastern Conference leaders New England Revolution were beaten 2-0 by New York City, who were fuelled by Valentin Castellanos' brace.
New York City equalled the longest home winning streak in club history, having also enjoyed a seven-game run from November 2017 to June 2018.
Nashville celebrated their first away victory of the season after taking down Atlanta United 2-0, DC United came from behind to defeat Philadelphia Union 3-1, Chicago Fire edged New York Red Bulls 1-0, Minnesota United topped Houston Dynamo 2-1 and Sporting Kansas City drew 1-1 with Colorado Rapids.
LAFC crushed the Whitecaps 6-0 at Banc of California Stadium on Wednesday, thanks to five goals in the opening 33 minutes.
The five goals scored in 32 minutes, 13 seconds was the fastest a team has netted five from kick-off in MLS history, according to Opta.
The Seattle Sounders had set the previous record (32:43) just 13 days ago against the San Jose Earthquakes.
LAFC went ahead in the second minute through Dejan Jakovic, who headed in a Brian Rodriguez corner.
Another corner led to Bradley Wright-Phillips finishing before the veteran forward produced another acrobatic effort in the 11th minute.
Things went from bad to worse for Vancouver when Ranko Veselinovic turned in a Rodriguez cross just three minutes later.
Diego Rossi made it 5-0 after a one-two with Mark-Anthony Kaye in the 33rd minute.
LAFC would have to wait until the second half for a sixth, Rodriguez's cross from the right leading to an Andy Rose own goal.
The win saw Los Angeles climb into sixth in the Western Conference, while Vancouver are ninth.
Orlando City extended their unbeaten run to seven games with a 2-1 win over Sporting Kansas City.
Tesho Akindele and Benji Michel scored before half-time and Orlando held on despite Johnny Russell's 53rd-minute strike.
The Earthquakes' struggles continued with a 5-0 loss to the Colorado Rapids.
San Jose have conceded five goals in a match five times this season, which is the most allowed by a team in a single campaign, according to Opta. The Earthquakes are just 13 games into their season.
Chicago Fire recorded a 4-0 win over the Houston Dynamo, while Inter Miami slumped to a 4-1 defeat against the New York Red Bulls.
History was made as Nashville beat DC United 1-0, with Tori Penso becoming the first female referee to oversee an MLS game in 20 years.
Columbus Crew retained their five-point lead atop the Eastern Conference thanks to a 2-1 win over Minnesota United.
The New England Revolution beat Montreal Impact 3-1, Toronto edged New York City 1-0 and Atlanta United overcame Dallas 1-0.
Cincinnati and Philadelphia Union played out a 0-0 draw, Real Salt Lake enjoyed a 2-0 win over the LA Galaxy and the Portland Timbers edged the Seattle Sounders 1-0.
LAFC entered Friday's contest having snapped a club-record four-match losing run with a 3-3 draw against LA Galaxy, though it extended the club-record winless streak (D3 L5) – the Los Angeles franchise had previously never gone more than five straight games without a win.
But Fall's double and goals from Cristian Arango and Eduard Atuesta ensured LAFC returned to winning ways at the expense of Sporting KC, who had lost just one of their last 12 MLS matches (W6 D5).
Rookie centre-back Fall opened the scoring in the 18th minute before doubling the lead just past the hour mark at Banc of California Stadium, where Sporting KC midfielder Roger Espinoza was sent off in the 58th minute.
Arango finished with authority to make it 3-0 with 19 minutes remaining and after Sporting KC's Ilie Sanchez had a goal ruled out for offside, Atuesta converted a penalty three minutes from the end.
LAFC are eighth in the Western Conference, just outside the play-off positions and 15 points behind leaders Seattle Sounders, while they are 13 points adrift of second-placed Sporting KC.
Elsewhere, Eastern Conference pacesetters and Supporters' Shield leaders New England Revolution edged Philadelphia Union 1-0.
Despite Arnor Traustason's red card in the second half, the Revolution stayed 14 points clear atop the standings after Nashville defeated New York City 3-1.
Portland Timbers were 2-0 winners over Houston Dynamo.
Though the visitors hit the lead twice in the first half at Exploria Stadium, the Lions equalised as many times, including a finely taken goal from Alexandre Pato in the 36th minute.
Sensing where the ball would drop following Mauricio Peyeyra's ball over and into the penalty area, Pato set himself and calmly volleyed past Maxime Crepeau.
Ilie Sanchez rifled home a mere six minutes into the second half and from there, LAFC were able to sit and absorb Orlando pressure.
After some important saves from Crepeau to deny a third equaliser, substitute Kwadwo Opoku was able to score an injury-time sealer in transition, moving Steve Cherundolo's side to four wins and a draw from five games.
Meanwhile, Philadelphia Union were able to keep their place atop the Eastern Conference, with a 2-0 win at home to Charlotte FC.
The Union took the lead after only four minutes and in uncomplicated fashion, with Sergio Santos running on to a ball over the top and assisting for Julian Carranza from the byline.
Daniel Gazdag doubled the margin a minute within the restart, and similarly to LAFC, absorbed pressure in their defensive half to see out the win to move to 13 points from five games.
NY Red Bulls and Atlanta stayed within three points of Philadelphia in the Eastern Conference with respective 1-0 victories over New England Revolution and DC United. Columbus Crew dropped points at home to Nashville, with Alex Muyl's lone goal the difference.
A Gonzalo Higuain penalty would not suffice for Inter Miami, who remain rooted to the bottom of the ladder in the East, after Phil Neville's side losing 3-1 to Houston Dynamo. Chicago Fire and FC Dallas played out a goalless draw, while Toronto FC beat reigning MLS Cup champions New York City 2-1.
In the Western Conference, Real Salt Lake fell two points behind with a 1-1 draw away to Colorado, Austin FC conceded two goals in as many minutes to eventually play out a 2-2 draw against San Jose Earthquakes.
Ryan Raposo's 73rd-minute winner was enough for Vancouver Whitecaps at home to Sporting KC, while Brent Kallman's own goal eventually condemned Minnesota to their first defeat of the season, losing 2-1 to the Seattle Sounders.
LAFC led twice in the first half at Exploria Stadium but were pegged back on both occasions, first by a finely taken goal from Alexandre Pato in the 36th minute.
Sensing where the ball would drop following Mauricio Peyeyra's delivery into the penalty area, Pato set himself and calmly volleyed past Maxime Crepeau.
But Ilie Sanchez rifled in a third LAFC goal six minutes into the second half and the visitors were able to absorb Orlando pressure thereafter.
After some important saves from Crepeau, substitute Kwadwo Opoku sealed victory in stoppage time, moving Steve Cherundolo's side to 13 points from five games after four wins and a draw.
Meanwhile, Philadelphia Union were also able to keep their place atop the Eastern Conference with a 2-0 win at home to Charlotte FC.
The Union took the lead in uncomplicated fashion after only four minutes, with Sergio Santos running onto a ball over the top and crossing for Julian Carranza from the byline.
Daniel Gazdag doubled the lead a minute into the second half, with that two-goal margin enough for the Union to see out the win and themselves move to 13 points.
New York Red Bulls and Atlanta United stayed within three points of Philadelphia in the East with respective 1-0 victories over New England Revolution and DC United. Columbus Crew lost at home to Nashville, with Alex Muyl's lone goal the difference.
A Gonzalo Higuain penalty counted for little for Inter Miami, who remain rooted to the bottom of the ladder in the East after Phil Neville's side lost 3-1 to Houston Dynamo. Chicago Fire and FC Dallas played out a goalless draw, while Toronto FC beat reigning MLS Cup champions New York City 2-1.
CF Montreal edged FC Cincinnati in a seven-goal thriller, trailing twice in the first half before recovering to win 4-3.
In the Western Conference, Real Salt Lake fell two points behind with a 1-1 draw away to Colorado Rapids, while Austin FC conceded two goals in as many minutes to draw 2-2 against San Jose Earthquakes.
Ryan Raposo's 73rd-minute winner was enough for Vancouver Whitecaps at home to Sporting Kansas City, while Brent Kallman's own goal ultimately condemned Minnesota United to their first defeat of the season, losing 2-1 to Seattle Sounders.
After three straight losses in all competitions, Los Angeles FC responded by thrashing the San Jose Earthquakes 5-1 at Banc of California Stadium.
Diego Rossi's beautiful finish into the corner opened the scoring after a poor turnover of possession by the Earthquakes in the 21st minute.
But LAFC had to wait until the second half to truly take control.
Another bad turnover allowed Bradley Wright-Phillips through to double the lead, before Jose Cifuentes made it 3-0.
Rossi added his second in the 69th minute before Danny Musovski made it 5-0, Danny Hoesen later adding a consolation goal for San Jose.
The win lifted LAFC up into third in the Western Conference, with San Jose sitting 11th.
The Galaxy made it three straight wins by edging the Portland Timbers 3-2 at Providence Park.
Efrain Alvarez opened the scoring for the Galaxy, completing a fine team move with a finish into the top corner from inside the area.
It took until five minutes into the second half for the visitors to double their lead through Cristian Pavon's individual effort.
Felipe Mora gave Portland hope with a 67th-minute goal, but Joe Corona struck for the Galaxy with a wonderful 20-yard strike.
Diego Valeri came off the bench to add a second for Portland in additional time.
The Timbers are sixth in the Western Conference, one spot behind the Galaxy while being level on points.
Columbus Crew returned to the top of the Eastern Conference after a 1-0 win over Philadelphia Union.
Lucas Zelarayan's deflected 25-yard effort in the 55th minute was enough for Columbus.
Elsewhere, Western Conference leaders Sporting Kansas City were held to a 1-1 draw by Dallas.
Real Salt Lake and the Seattle Sounders drew 2-2 in a clash that featured a screamer from Pablo Ruiz, while Orlando City played out a 1-1 draw with Nashville.
New York City overcame New England Revolution 2-0, Houston Dynamo beat Minnesota United 3-0 and Erik Sorga scored a 98th-minute winner for DC United as they edged the New York Red Bulls 1-0.
Atlanta United and Inter Miami drew 0-0, while Cincinnati's clash with Chicago Fire also finished goalless.
Montreal trailed 2-0 after 14 minutes and 4-2 after 46 minutes, but Ahmed Hamdi scored twice in the final 16 minutes to give the home side a wild victory.
In Nashville, Hany Mukhtar scored in the 10th, 13th and 16th minutes on the way to a 5-1 rout of Chicago Fire.
The six-minute span was the second-shortest for a hat-trick in MLS history after LA Galaxy's Harut Karapetyan needed just five minutes to score three in a 1998 match.
Gustavo Bou's team-leading seventh goal gave New England Revolution a 1-0 win at Atlanta United, snapping a three-game win drought for the Eastern Conference leaders.
Second-half goals from Deiber Caicedo and Cristian Dajome gave Vancouver just their third win from 13 games this season, a 2-1 triumph over LA Galaxy.
Darlington Nagbe's impressive individual effort opened the scoring in a 2-1 Columbus Crew win over New York City FC.
Nani's 77th-minute penalty gave Orlando City a 1-1 draw with Toronto after Jozy Altidore had opened the scoring five minutes earlier, while Cole Bassett's second-half goal for Colorado Rapids earned a 1-1 draw against San Jose.
Kacper Przybylko's 83rd-minute decider gave Philadelphia Union a 2-1 victory over DC United, while Jeremy Ebobisse scored in the 84th minute to lift Portland past Dallas 1-0.
Carlos Vela's left-footed rocket in the 79th minute was the difference in LAFC's 2-1 home win over Real Salt Lake.
The New York Red Bulls-Inter Miami match was postponed due to weather.
Columbus made it five wins in six MLS games this season with a 3-0 victory over Chicago Fire on Thursday.
Gyasi Zardes scored and also missed a penalty as Darlington Nagbe scored a stunner at MAPFRE Stadium.
Derrick Etienne found the bottom corner from a Milton Valenzuela pass to open the scoring for Columbus in the 20th minute.
Zardes put a penalty over the crossbar just before half-time and Columbus left it late to seal their win.
Nagbe flicked the ball up to himself 30 yards from goal before unleashing a stunning volley into the corner to make it 2-0.
Zardes sealed the win in the 88th minute, tapping in Pedro Santos' cross as the Crew moved four points clear atop the conference.
The Hudson River Derby went the Red Bulls' way as they recorded a 1-0 win over New York City.
Kyle Duncan's powerful 20-yard strike just before the hour-mark was spilled by City goalkeeper Sean Johnson and awarded after a VAR review.
The Red Bulls are third in the Eastern Conference, while New York City are second last.
In the day's other game, New England Revolution and Philadelphia Union drew 0-0.
Former Manchester United attacker Nani scored and provided an assist in Orlando's 4-1 thrashing of the Chicago Fire on Saturday.
After Chris Mueller opened the scoring, he crossed for Nani to head in a second in the 24th minute.
Given a chance to get back into the game, Chicago squandered it when Alvaro Medran had a penalty well saved by Pedro Gallese.
Chicago took their next chance from the spot through Robert Beric early in the second half, but Orlando sealed their win late.
Junior Urso scrambled in from inside the area after Nani's set-piece went uncleared, and the Portuguese attacker's pass later found Benji Michel to finish off in additional time.
Orlando are second in the Eastern Conference, five points behind the high-flying Columbus Crew.
Second-half goals from Pedro Santos and Gyasi Zardes saw Columbus record a 2-0 win over Nashville.
Diego Valeri netted a brace as the Portland Timbers crushed the San Jose Earthquakes 6-1 at Avaya Stadium.
Fresh off confirming the signing of Gonzalo Higuain, Inter Miami recorded a second win in three matches by beating Atlanta United 2-1.
Elsewhere, a 10-man LA Galaxy fell to the Colorado Rapids 2-0 and Dallas also finished with 10 but recorded a 3-2 win over Sporting Kansas City.
Haris Medunjanin scored directly from a corner in the 85th minute as Cincinnati beat the New York Red Bulls 1-0 and the Vancouver Whitecaps beat a 10-man Real Salt Lake 2-1.
New York City drew 0-0 with the New England Revolution, DC United and Toronto played out a 2-2 draw, while the Houston Dynamo and Minnesota United did likewise.
Former Manchester United winger Nani scored his ninth goal of the MLS season as Orlando City missed the chance to close the gap at the top after a 1-1 draw at Cincinnati on Saturday.
Cincinnati, who were winless in their past seven games coming in, opened the scoring in the 42nd minute from Brazilian attacker Brenner.
Nani levelled moments after being brought into the game as a 55th-minute substitute, driving forward and firing in a low left-foot pinpoint shot.
The result means back-to-back draws for Orlando who are second in the Eastern Conference, seven points behind leaders New England.
Third-placed New York City blew an early two-goal lead to draw 2-2 away to Toronto.
Santiago Rodriguez and Ismael Tajouri had put City up 2-0 inside 21 minutes but Toronto netted twice after the break.
Richie Laryea pulled one back in the 49th minute before reigning MVP Alejandro Pozuela equalised in the 55th minute with a tap in.
Portland Timbers beat Real Salt Lake 3-2 in a see-sawing contest with Felipe Mora netting the eventual winner.
Columbus Crew suffered their third straight defeat as Ezequiel Barco netted a double for Atlanta City in a 3-2 win.
Dallas continued their good run of recent form, claiming their third win in four games, with a 2-0 triumph over Austin.
Minnesota United won 2-0 over Houston Dynamo, while Colorado Rapids drew 0-0 with Western Conference leaders Sporting KC.
Tesho Akindele scored the fastest goal in club history as Orlando got off to a quick start at home to Jaap Stam's Cincinnati on Saturday.
Akindele found the back of the net after just 33 seconds, surpassing the Orlando record set by Chris Mueller (62 seconds) in April 2018.
Nani doubled the lead in stunning fashion in the 19th minute – the former Manchester United star leaving Yuya Kubo on the floor after cutting to his right foot and then his left as he curled the ball past Przemyslaw Tyton.
After Nani hit the post minutes later and saw his shot saved by Cincinnati goalkeeper Tyton just past the hour mark, Junior Urso sealed the victory with 10 minutes remaining.
Orlando – who remain unbeaten through three games – are third in the Eastern Conference, behind leaders New England Revolution and second-placed New York City.
The Revolution edged Atlanta United 2-1 and New York City defeated reigning Supporters' Shield winners Philadelphia Union 2-0.
The Earthquakes and Dallas, meanwhile, both won 4-1 against DC United and Portland Timbers respectively.
Jackson Yueill's brace and a pair of assists from Cade Cowell fuelled the Earthquakes, who are top of the Western Conference on goal difference but level on points with Real Salt Lake, LA Galaxy and Austin FC.
Dallas showed no mercy at home to the Timbers after Andres Ricaurte and Jader Obrian scored in the opening 13 minutes.
Real Salt Lake came from behind to top Sporting Kansas City 3-1 courtesy of Rubio Rubin's second-half double, while MLS newcomers Austin prevailed 1-0 against Minnesota United.
Elsewhere, New York Red Bulls were 2-0 winners over Chicago Fire, defending champions Columbus Crew were held to a goalless draw by Montreal Impact and Houston Dynamo and Los Angeles FC played out a 1-1 draw.
Bruce Arena's side, who clinched their first-ever Supporters' Shield on Saturday, won thanks to Tajon Buchanan's 74th-minute strike.
The result took Revolution's season tally to 73 points from 33 games, eclipsing the previous record mark held by LAFC with 72 points in 2019.
New England have one more regular season match to extend their record, playing Inter Miami at home on November 7.
Canada international Buchanan came up with the winner, showing fast feet to make space for a low shot that beat Rapids goalkeeper William Yarbrough, who had sensationally kept out Henry Kessler from close range minutes earlier.
New York Red Bulls slipped out of the Eastern Conference play-offs spots after a 1-0 defeat to DC United who moved above them into seventh place.
United snapped a four-game winless skid, while also ending the Red Bulls' four-game running run and eight-game unbeaten streak in the process. Kevin Paredes side-footed home the ninth-minute winner for United.
Sporting KC moved into top spot in the Western Conference and dented LA Galaxy's play-offs aspirations with a 2-0 win.
Johnny Russell extended his scoring run to eight straight games with both goals for KC who clinched a top-four seeding as well.
Randall Leal scored two goals and grabbed an assist as Nashville came from two goals down twice to win 6-3 at Cincinnati.
Portland Timbers moved in the west's top four with a 2-0 home victory over San Jose Earthquakes, while Vancouver Whitecaps climbed up to fifth with a 2-1 triumph over Minnesota United.
New York City boosted their Eastern Conference top-four seed hopes with a 1-0 home win over Chicago Fire, while Josef Martinez's winner helped Atlanta United past Inter Miami 2-1 after Gonzalo Higuain had opened the scoring from the spot.
Columbus Crew upset Orlando City 3-2, Toronto held Philadelphia Union 2-2 as Jozy Altidore netted while Real Salt Lake scored twice in the final 10 minutes to win 2-1 at Dallas.
New England sensationally trailed 3-0 within half an hour against lowly Toronto after early strikes from Yeferson Soteldo, Kemar Lawrence and Tsubasa Endoh.
Japanese midfielder Endoh's strike was spectacular, cutting inside before lashing a fine curling shot home in the 24th minute.
Revolution came into the game after stumbling in their past two games, with a draw and a loss following six straight victories and the defeat extended that winless run.
New England fought in the second half, with Spanish midfielder Carles Gil pulling one back in the 56th minute.
In the 78th minute it was 3-2 when Ghanaian Emmanuel Boateng laid off for Adam Buksa to net his fourth of the season, but there was no late equalizer.
New England's lead in the east remains three points as second-placed Orlando City lost 3-1 to Chicago Fire who claimed back-to-back wins.
Seattle Sounders returned to winning ways and extended their unbeaten season with a 2-0 home victory over Houston Dynamo.
Raul Ruidiaz netted his 10th goal of the campaign in second-half stoppage time after Kelyn Rowe put Seattle ahead just past the hour.
The undefeated Sounders have 29 points from 13 games and are three points clear of the west's second-placed Sporting Kansas City.
LA Galaxy are third in the Western Conference, aided by a 3-1 home triumph over Dallas despite the absence of top scorer Javier 'Chicharito' Hernandez.
Rayan Raveleson, Kevin Cabral and Ethan Zubak netted for the Galaxy, who were without Hernandez to a calf injury.
Real Salt Lake inflicted more pain on struggling Vancouver Whitecaps with a 4-0 road win with Damir Kreilach netting a brace, while Colorado Rapids got past Minnesota United 2-0.
Montreal extended their unbeaten run to five games after Romell Quioto's 73rd-minute winner in a 2-1 victory over New York City, while LAFC moved up to fifth in the Western Conference with a 2-0 win at Austin.
The Revolution improved their record, with back-to-back wins, to the best in the MLS with 30 points from 15 games, sitting atop the Eastern Conference.
Inter are bottom of the east, with only eight points from 14 games, having lost their past six.
Carles Gil provided the assist, his 11th of the season, for Traustason's 15th-minute header, before Teal Bunbury fired in a 27th minute goal from Inter goalkeeper Nick Marsman's parry.
Icelandic midfielder Traustason grabbed his second in the 36th minute, whipping in a shot after a goalmouth scramble from a corner.
Gil was crafty on the right side of the box before setting up another goal with Adam Buksa lashing home a fine volley on the stroke of half-time.
Buksa grabbed a double in the 83rd minute as Gustavo Bou put him into space with the Polish striker finishing with class.
Without Javier Hernandez, LA Galaxy came from two goals down to claim a 2-2 draw away to Real Salt Lake.
Albert Rusnak and Maikel Chang had scored inside the opening 25 minutes to give RSL a 2-0 lead.
Victor Vazquez pulled one back with a top-corner strike in the 33rd minute, before RSL keeper David Ochoa had spectacularly saved a second-half Rayan Raveloson header.
But Madagascar talent Raveloson headed in a 77th-minute equaliser to earn a share of the spoils.
DC United scored twice in the final 10 minutes to rescue a 2-2 draw at struggling Chicago Fire.
Gaston Gimenez's 32nd-minute strike, on his return from the Copa America, and Andy Najar's 61st-minute own goal had Chicago on track but United hit back late.
In the 82nd minute, Yordy Reyna laid off for Kevin Paredes who fired in off the post, before Ola Kamara converted an 87th-minute penalty.
Hungarian forward Daniel Salloi netted a 94th minute equalizer for high-flying Sporting KC to claim a 1-1 home draw with 10-man San Jose Earthquakes who are winless in 10.
Salloi forced home a rebound after Johnny Russell's free-kick hit the woodwork after Nathan had headed the Earthquakes ahead from a corner against the run of play early in the second half.
Felipe Mora glanced on a late winner for Portland Timbers to edge Los Angeles FC 2-1 after Carlos Vela had earlier squared up following Diego Valeri's 100th goal for the club.
Colorado Rapids eased past Dallas 2-0 to extend their unbeaten run, Cincinnati drew 1-1 with Atlanta United and Columbus Crew and Nashville played out a goalless draw.
Toronto drew 1-1 with New York Red Bulls, while New York City edged Montreal 1-0.
After failing to score in their opening two games – a 1-0 loss to LA Galaxy and a goalless draw with Vancouver Whitecaps – City hit the lead after only seven minutes, with Alexander Callens rifling home after Montreal failed to clear their lines from a corner.
Santiago Rodriguez spectacularly chipped Sebastian Breza to double the margin on 20, but only after Montreal gifted City possession in an attempt to play out from their own half.
Zachary Brault-Guillard pulled one back for CFM, but following further City goals from Telles Magno and Thiago Andrade, the scoreline arguably belied the performance.
Douglas Costa opened his account in the MLS later on Saturday, scoring the equaliser in LA Galaxy's 3-2 loss away to Seattle Sounders.
Javier Hernandez gave the Galaxy a shock lead in the sixth minute, after fine link-up play between Raheem Edwards and Kevin Cabral, but the Sounders fought back to lead before the interval – with goals from Jordan Morris and Fredy Montero.
Costa drew the visitors level from the dead ball after 48 minutes, wrong-footing Stefan Frei, but Xavier Arreaga headed home in the 72nd to give the Sounders their first win of the season.
Los Angeles FC continued their unbeaten start to the season, meanwhile, grinding out a 2-0 win against a 10-man Inter Miami at DRV PNK Stadium.
A deflected Carlos Vela pass put Kwadwo Opoku through on the stroke of the interval, and the 20-year-old Ghanaian finished with composure. That didn't spell the end of events in the first half, though, as Brek Shea was given a straight red card in injury time.
LAFC had six shots for the match compared Inter's seven, despite 53 per cent possession and an extra man. However, an Ismael Tajouri free-kick crept inside Inters back post in the 82nd minute to secure the win.
Steve Cherundolo's side are equal top in the Western Conference with Real Salt Lake, who defeated New England Revolution 3-2. Austin FC couldn't keep pace in the final Saturday game, however, going down 1-0 away to Portland Timbers through a Bill Tuiloma goal.
Columbus Crew share the lead in the Eastern Conference, following their 2-1 win over Toronto FC. They are joined by Philadelphia Union, who defeated San Jose Earthquakes 2-0.
A Darwin Quintero brace handed Houston Dynamo a 2-0 win against Vancouver Whitecaps, while Brandon Vazquez scored a double of his own in FC Cincinnati's 2-1 win at Orlando City.
FC Dallas, Chicago Fire and Colorado Rapids respectively secured 2-0 victories over Nashville, DC United and Sporting Kansas City in Saturday's other games.
Tesho Akindele's header after 12 minutes to give Orlando City a 1-0 victory against Toronto FC, giving the Florida side three wins and three draws through six matches.
The Revolution saw off 10-man New York Red Bulls 3-1 behind first-half goals from Gustavo Bou and Tajon Buchanan and an Adam Buksa tally late to seal it.
Two late strikes from Lucas Zelarayan helped the Columbus Crew stun New York City FC 2-1 after the home side led into the 82nd minute.
Felipe Mora's second-half brace and a penalty from Diego Valeri lifted Portland to a 3-0 triumph over 10-man LA Galaxy.
Diego Rossi scored twice before the break as Los Angeles FC held on to defeat Colorado Rapids 2-1.
Houston's Memo Rodriguez and Maximiliano Urruti provided the winning margin for Houston as they won 2-1 over Vancouver.
Sporting Kansas City continued their strong start, shaking off an early San Jose Earthquakes goal from Javier Lopez en route to a 3-1 win as Jaylin Lindsey, Alan Pulido and Daniel Salloi found the back of the net.
Luka Stojanovic lifted Chicago Fire past Inter Miami 1-0 for their first win of the season, and FC Cincinnati also emerged victorious for the first time this term with a 2-1 win at Montreal.
Real Salt Lake escaped with a 2-2 draw at FC Dallas thanks to Damir Kreilach's goal after 86 minutes.
