Tom Curry will not return before the end of the Six Nations due to another hamstring injury.
Curry had been in line to return for England against Wales this week after missing time with a tear to his right hamstring.
But after making his comeback for Sale Sharks on Saturday, he injured the same muscle in his left leg.
On Tuesday, England confirmed Curry would be replaced in the squad by brother Ben.
An update followed from Sale director of rugby Alex Sanderson, who ruled the back rower out for the remainder of the tournament.
"It's a grade 2c hamstring injury on the opposite side to the one that he did six weeks ago," Sanderson said.
"It's a soft muscle injury that extends into the tendon. It's four to six weeks."
Sanderson explained the injury had been sustained after linking up with England this week rather than in the club match against Northampton Saints.
He attributed the setback to "something to do with the loading and the physical demands of an international camp compared to your club".
Defending the decision to involve Curry against Northampton, Sanderson said: "Tom knows his body and his mind better.
"This is not a guy who needs two or three games to warm up. He can just go straight in and be at the top of his game."
Princess of Wales visits Maidenhead Rugby Club – Wednesday’s sporting social
Sports stars and clubs across the world continue to provide an insight into their lives on social media.
Here, the PA news agency looks at some of the best examples from June 7.
Rugby Union
A royal visit to Maidenhead Rugby Club.
Cricket
There was excitement around Moeen Ali’s decision to answer England’s Ashes call.
Stuart Broad likes wine.
Football
Pants malfunction.
Mesut Ozil marked his wedding anniversary.
Leah Williamson took over the camera.
Wayne Rooney promoted Soccer Aid.
Tom Cairney accepted his award.
Tennis
Serena Williams and her daughter posed for the camera.
Boxing
Tyson Fury was hard at it.
London Irish file for administration after suspension from all club competitions
London Irish have filed for administration following their suspension from all club competitions.
The Rugby Football Union on Tuesday confirmed that neither Irish’s owner Mick Crossan nor the American consortium planning to buy the club were able to prove they had the finances to compete in the Gallagher Premiership for the 2023-24 campaign.
And Crossan has revealed that entering administration was the only choice left after the suspension ended any chance of the prospective takeover going through.
Crossan said: “This decision has ultimately ended any hope of an acquisition of the club and has regrettably forced us to file for administration this morning (Wednesday).
“Administration has always been the last resort and something we hoped we could avoid. And we bitterly regret the difficulties it will present to each and every one of you.
“My focus is now on working with the appointed administrator and I hope that the club will come out of administration as quickly as possible.
“As a lifelong fan of London Irish, the club’s suspension is bitterly disappointing and I understand the sadness felt by the thousands of our loyal supporters and the frustrations of our incredible coaches, medical staff, back-office team, and players.”
Moeen Ali agrees to come out of retirement to join England’s Ashes squad
Moeen Ali has answered England’s Ashes call, reversing his retirement from Test cricket for one last mission against Australia.
England’s plans for the series were derailed by a stress fracture to first-choice spinner Jack Leach but Moeen has agreed to return to red-ball cricket for the first time in almost two years to fill the breach.
The 35-year-old was invited to join the squad at the start of the week and, after taking a couple of days to ponder his decision, has now agreed to join up with the team.
England did have other options, including 18-year-old Rehan Ahmed, who became England’s youngest Test cricketer when he debuted in Karachi in December, and Surrey’s Will Jacks but none with the experience and credentials of Moeen.
He has 64 Test caps, 195 wickets and the small matter of five Test centuries. With Jonny Bairstow slotting back into the side at number seven following his return from a broken leg, Moeen is likely to bat at eight and give the home side a seriously dangerous lower order.
The Ashes begins at Moeen’s home ground of Edgbaston on July 16, with the all-rounder due to turn 36 on day three.
Rob Key, England’s managing director of men’s cricket, was involved in discussions alongside head coach Brendon McCullum and captain Ben Stokes and explained: “We reached out to Mo early this week about returning to Test cricket. Having had a couple of days to reflect, Mo is excited to join the squad and play Test cricket again.
“His vast experience, along with his all-round ability, will benefit our Ashes campaign. We wish Mo and the rest of the squad well for the Ashes campaign.”
McCullum previously tried to tempt Moeen back into the fray for the historic tour of Pakistan but, after initially declaring himself open to the idea, Moeen ultimately decided to stick to limited-overs cricket.
Speaking at the time, Moeen concluded: “I want to enjoy my cricket and it wouldn’t be fair to reverse my decision and then struggle to give it my all. It’s time to close the door on that side of my career. To play 64 Tests for England has been a privilege and a dream fulfilled.”
He has now been persuaded to take the field again, warming to the idea of turning out for Stokes and McCullum. They have overhauled the way England play the game, prioritising an attacking, proactive style that fits Moeen’s own approach like a glove.
Australia have proved thorny opponents for Moeen in the past, with his career bowling average climbing from 36.66 to 64.65 in Ashes cricket, but he is ready to embrace unfinished business against England’s biggest rivals.
He will need to get back to grips with the red Dukes ball after dedicating himself to the white-ball formats since September 2021, and will work alongside spin coach Jeetan Patel in the run-up to the series opener in Birmingham.
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