Emma Raducanu retired from her first-round match with Bianca Andreescu at the Internazionali d'Italia because of a lower back injury.
The world number 12 was trailing 6-2 2-1 when calling time on the match against Andreescu in a highly anticipated showdown between two of the past three US Open winners.
Raducanu initially left the court for treatment ahead of the seventh game of the opening set for a medical timeout and completed just four more games.
Speaking after her withdrawal, coming two weeks before the French Open, Raducanu explained she did not want to aggravate an injury sustained at last week's Madrid Open.
"I thought maybe taking one, two days off, it would go away because a lot of the other small niggles I've had, they've kind of gone away after taking, like, two days off," she said.
"Then I got here and I was training, but it just didn't seem to get better. I was training with some limitations. I wasn't moving really.
"I was just playing where I knew where the ball was coming, just staying in one corner. I think I must have underestimated the unpredictability of competition in a match.
"The last few weeks have been really positive. I've learned a lot about myself and my game has definitely improved on this surface but I need to make sure my back is fully right.
"I need to just keep on it. I don't want to play my next match with a feeling of limitation because I think that I learned my lesson from this week, when to push, when not to push."
Ons Jabeur joined Andreescu in the last 32 with a 6-0 7-6 (7-1) victory over Sorana Cirstea to keep her impressive run of form going.
The Tunisian won her first WTA 1000 title in Madrid last week and has now won seven successive main draw matches for the first time in her career
Elsewhere in Tuesday's action, American qualifier Lauren Davis pulled off a shock 6-2 6-3 win against number 11 seed Jelena Ostapenko.
Fellow Americans Jessica Pegula, Madison Brengle and Amanda Anisimova also advanced, beating Liudmila Samsonova, Marta Kostyuk and Tereza Martincova respectively.
Anisimova, who is now the player with the most wins in three sets so far in 2022 with nine, will face Belinda Bencic for a place in the last 16.
French Open day seven: Coco Gauff wins teenage battle as Iga Swiatek marches on
Coco Gauff fought off the challenge of 16-year-old Mirra Andreeva to reach the fourth round of the French Open.
Gauff came back from a set down to defeat the Russian 6-7 (5) 6-1 6-1 while Iga Swiatek hammered Wang Xinyu 6-0 6-0, but Elena Rybakina was forced to withdraw because of illness.
In the men’s event, Casper Ruud and Holger Rune are on track for a quarter-final meeting, while Alexander Zverev defeated Frances Tiafoe in the night match.
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Cameron Norrie’s third-round exit completed a miserable French Open for Britain’s senior singles players but, after several years of very little junior presence, there are six British competitors in the main draws of the boys’ and girls’ singles, including 14-year-old qualifier Hannah Klugman.
Fallen seeds
Men: Taylor Fritz (9), Frances Tiafoe (12), Borna Coric (15)
Women: Elena Rybakina (4), Ekaterina Alexandrova (23)
Who’s up next?
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Novak Djokovic will hope to have a more comfortable afternoon than against Alejandro Davidovich Fokina when he takes on Juan Pablo Varillas in the fourth round.
Carlos Alcaraz meets Lorenzo Musetti while the first women’s night session of the year sees Australian Open champion Aryna Sabalenka meet former finalist Sloane Stephens.
Fifth seed Stefanos Tsitsipas will bid to keep his strong form going against Sebastian Ofner while Elina Svitolina meets Russian Daria Kasatkina.
Paris Saint-Germain 2-3 Clermont: Messi and Ramos denied winning send-off as visitors fight back
Paris Saint-Germain failed to muster a winning send-off for Lionel Messi and Sergio Ramos as they ended the Ligue 1 season by squandering a two-goal lead in a 3-2 loss against Clermont.
Early goals for the Spaniard and Kylian Mbappe put the champions on top at Parc des Princes, before Johan Gastien, Mehdi Zeffane and Grejohn Kyei completed a stirring comeback from the visitors.
It was a damp last note for ex-LaLiga heroes Messi and Ramos, who leave Paris as two-time league champions but without having won supporters' hearts – with Messi booed ahead of kick-off.
Likewise, it may prove a dour final chapter for coach Christophe Galtier, who is widely tipped to depart despite leading PSG to a record 11th league title.
PSG, who wore shirts adorned with the name of injured team-mate Sergio Rico in the first half, needed VAR to rescue them after Kyei prodded home an early finish.
The hosts promptly seized control, with Ramos netting a farewell header from Vitinha's delivery before Mbappe doubled their lead with an excellent penalty following Alidu Seidu's foul on Achraf Hakimi.
Clermont refused to fold though, with Gastien capitalising on Marco Verratti's mistake to halve the arrears and Zeffane bundling in a leveller either side of Kyei putting a spot-kick wide in a thrilling first half.
Messi, who misfired on his farewell, spurned a chance to finish Mbappe's square ball after the restart before Kyei nosed the visitors in front with a close-range finish.
PSG continued to toil for an equaliser in the closing stages and Messi almost stole a point in the final minute of injury time, only for goalkeeper Mory Diaw to stop his direct free-kick with an acrobatic save.
RB Leipzig 2-0 Eintracht Frankfurt: Nkunku and Szoboszlai strikes ensure DFB-Pokal defence
Christopher Nkunku and Dominik Szoboszlai scored as RB Leipzig retained the DFB-Pokal with a hard-fought 2-0 win over Eintracht Frankfurt in Saturday's final at the Olympiastadion.
Nkunku – playing what will likely be his final game for Leipzig amid suggestions he is set to join Chelsea – beat Kevin Trapp with a deflected effort 71 minutes into a tight encounter in Berlin.
Leipzig were rarely threatened by an uninspired Eintracht team, and Marco Rose's men wrapped up the victory when Nkunku turned provider, teeing up Szoboszlai to score five minutes from time.
That meant Leipzig capped a third-placed Bundesliga finish with a second major trophy in as many seasons, while Oliver Glasner's final game in charge of Eintracht was one to forget.
Timo Werner spurned a glorious chance to put Leipzig ahead after four minutes, side-footing straight at Trapp following a lightning counter-attack.
Randal Kolo Muani hit the side netting from a tight angle and Nkunku forced Tuta into a last-ditch block at the other end, but both sides struggled to break their opponents down in a cagey first half.
Eintracht's threat grew after the interval, but Mario Gotze volleyed straight at Janis Blaswich when presented with their best sight of goal, and Glasner's side were swiftly punished.
There was a huge stroke of fortune as Nkunku struck first, the Frenchman cutting in from the left before seeing his shot take two deflections to beat the unfortunate Trapp.
Eintracht never looked like fighting back from there, though, and Leipzig put the result beyond all doubt when Szoboszlai fired into the bottom-left corner after composed play by Nkunku.
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