Daniil Medvedev headlines the list of Russian and Belarusian players who will be banned from competing at Wimbledon this year.
In the wake of Russia's invasion of Ukraine, which was facilitated by Belarus, the four grand slams and the ATP and WTA Tours initially confirmed Russian and Belarusian players would be able to continue playing, albeit under neutral flags.
However, the All England Club has now decided athletes from the two nations will be unable to feature at the season's third grand slam.
That means reigning US Open champion Medvedev, ranked second in the world by the ATP behind Novak Djokovic, will not be involved.
With Medvedev a doubt for the French Open having undergone hernia surgery, he could miss two of this year's majors. He has never had much success at Wimbledon, with his best run ending in the fourth round in 2021.
WTA world number four Aryna Sabalenka, who hails from Belarus, is another big name to miss out, along with Russian ATP world number eight Andrey Rublev, who has won two titles so far in 2022.
Russian women's number one Anastasia Pavlyuchenkova, compatriot and 2018 Wimbledon quarter-finalist Daria Kasatkina and Belarusian two-time All England Club semi-finalist Victoria Azarenka will all also be absent.
"We share in the universal condemnation of Russia's illegal actions and have carefully considered the situation in the context of our duties to the players, to our community and to the broader UK public as a British sporting institution," a statement on the official Wimbledon website read.
"We have also taken into account guidance set out by the UK Government specifically in relation to sporting bodies and events.
"In the circumstances of such unjustified and unprecedented military aggression, it would be unacceptable for the Russian regime to derive any benefits from the involvement of Russian or Belarusian players with The Championships. It is therefore our intention, with deep regret, to decline entries from Russian and Belarusian players to The Championships 2022."
Chairman of the All England Club, Ian Hewitt, said: "We recognise that this is hard on the individuals affected, and it is with sadness that they will suffer for the actions of the leaders of the Russian regime.
"We have very carefully considered the alternative measures that might be taken within the UK Government guidance but, given the high profile environment of The Championships, the importance of not allowing sport to be used to promote the Russian regime and our broader concerns for public and player (including family) safety, we do not believe it is viable to proceed on any other basis at The Championships."
Wimbledon's statement confirmed that the ban would be "reconsidered" should circumstances change by June.
The move comes a month after UK sports minister Nigel Huddleston warned Medvedev and other Russian athletes they might be banned from Wimbledon unless they denounced president Vladimir Putin.
Medvedev and Rublev both called for peace in the immediate aftermath of Russia's attack on Ukraine.