Two-time Grand Slam winner Simona Halep's return to the WTA Tour ended in defeat to American Danielle Collins in almost three hours in the National Bank Open in Montreal on Wednesday.
World number 28 Collins triumphed 2-6 6-4 6-4 over the sixth seed Romanian, with the second-round match lasting two hours and 55 minutes.
Halep, who is a two-time winner in Montreal, was returning to the Tour for the first time in three months since suffering a calf tear against Angelique Kerber in Rome.
Collins, who has won titles recently in San Jose and Palermo, loomed as a tough first-up test for Halep, who broke twice to win the first set 6-2 as she generated eight break points to one.
The game turned from then on, as Collins sent down 4-1 aces in the second set and finished with 38 winners for the match.
Tokyo 2020 bronze medalist and third seed Elina Svitolina was bundled out in a second-round shock, losing 3-6 6-3 6-2 to Briton Johanna Konta.
Svitolina boasted a 5-0 record against Konta heading into the match but was beaten in a two-hour-and-11-minute struggle.
Top seed Aryna Sabalenka defeated former U.S. Open champion Sloane Stephens in three sets to secure her third-round spot.
Sabalenka fought back from the brink, rallying to win the last five games and triumph 7-6 (7-4) 4-6 6-4 over the American wildcard in a two hours and 25 minutes.
Fourth seed Karolina Pliskova came from behind to win 4-6 6-3 7-6 (7-2) over Donna Vekic, while Jessica Pegula also fought back after a slow start to topple 10th seed Anastasia Pavlyuchenkova 1-6 6-3 6-2.
Greek 11th seed Maria Sakkari won in three sets 6-4 6-7 6-4 over Veronika Kudermetova, while 13th seed Ons Jabeur responded after a lopsided first set to win 2-6 6-3 6-4 over Daria Kasatkina.
Cori Gauff progressed after being 5-0 up as Anastasia Potapova retired, while Amanda Anisimova, Camila Giorgi, Rebecca Marino and Sara Sorribes Tormo all won too.
Canadian local hope Marino will take on top seed Sabalenka, while Gauff faces Svitolina's conqueror Konta.