A further 25 players have been forced into hard lockdown for two weeks prior to the Australian Open, tournament organisers have confirmed.
Tennis' season-opening grand slam was plunged into crisis on Saturday when it was announced 47 players would be consigned to their hotel rooms for 14 days and not eligible to practise.
Officials said the protocols were as a result of two passengers testing positive for coronavirus on a flight from Los Angeles that arrived on Friday morning, along with another passenger who flew in from Abu Dhabi.
That affected 24 players aboard the LA flight and 23 on the plane from Abu Dhabi, while another positive test for a passenger arriving in Melbourne from Doha on Saturday morning has taken the total number of players affected to 72.
A statement from the Australian Open read: "One positive COVID-19 test has been returned from a passenger on a charter flight into Melbourne from Doha which arrived at 5.30am on January 16.
"The passenger is not a member of the playing contingent and had tested negative before the flight.
"There were 58 passengers on the flight, including 25 players. All are already in quarantine hotels.
"The 25 players on the flight will not be able to leave their hotel room for 14 days and until they are medically cleared. They will not be eligible to practise."
About 1,200 players and staff have been arriving in Melbourne on sparsely populated aeroplanes ahead of the delayed Australian Open, which is due to get under way on February 8.
Speaking on Saturday, Tennis Australia CEO Craig Tiley insisted the tournament would be going ahead despite the chaos and the lack of preparation time for many of the playing contingent.
"It's not something we wanted to happen," he told The Today Show. "We were hoping every flight would be okay. We're in this situation, we have to deal with it.
"The Australian Open is going ahead and we'll continue to do the best we can possibly do to ensure those players, who are not in a great position, find it somewhat acceptable.
"We're planning on February 8, we do have that buffer time in there. We're looking forward to welcoming fans to the Australian Open.
"Ticket sales have been going well, we've got two weeks of great tennis and our intention is to continue with those dates."
Nadal unsure of future involvements after Laver Cup withdrawal
Rafael Nadal says he cannot commit himself to future tournaments after his withdrawal from the Laver Cup.
Nadal shared news on Thursday that he would not make an appearance at the annual event in Berlin, stating that it would not be in the best interests of Team Europe if he were to participate.
The Spaniard was last in action playing doubles at the Paris Olympics with Carlos Alcaraz, and while he says he is in a good space mentally, he was not able to confirm when he would next make a professional appearance on a tennis court.
He said: "Mentally, I’m good, no problems. I'm at home enjoying other things, training as much as I can every day.
"No drama, obviously these are decisions [the Laver Cup withdrawal] that are being made, and this was a possibility - it's been a while without competing. There will be someone else who can do better.
"Right now I’m not in a position to put myself down for anything.
"I said [I would play] until the Olympics and then we would see, and I'm in that period of rest."
Nadal has endured a difficult 2024 so far, playing just 21 competitive singles matches with a record of 13-8.
He has only one grand slam victory to his name this year, beating Jack Draper in the Australian Open, but he lost in the first round of the French Open to eventual finalist Alexander Zverev.
Alcaraz sends Spain into Davis Cup last eight
Carlos Alcaraz has led Spain to the quarter-finals of the Davis Cup Finals after a straight-sets win over Ugo Humbert.
The 21-year-old needed just one hour and 21 minutes to cruise through with a 6-3 6-3 victory, hitting 21 winners to his opponent's 14 as he backed up Roberto Bautista's win earlier on.
Bautista had to come from behind to edge past Arthur Fils, beating him 2-6 7-5 6-3, and converting 40% of his break points, bettering the Frenchman's 27%.
Spain are through in Group B, but elsewhere in Group D, US Open semi-finalist Jack Draper's return was unable to inspire Great Britain to victory.
Dan Evans was beaten by Tomas Martin Etcheverry earlier on Friday before Draper's first match since New York ended in a straight-sets 7-6 (7-4) 7-5 defeat to Francisco Cerundolo.
Despite making fewer unforced errors than the Argentinian (23 to 39), the British number one struggled to find an edge, though Evans and Neal Skupski claimed a consolation win in the doubles to make it 2-1.
They sit second in the group, missing the chance to qualify for the last eight, and now needing a win against Canada, who have been in fine form so far.
Auger-Aliassime and Shapovalov sweep aside Finland at Davis Cup
Felix Auger-Aliassime and Denis Shapovalov moved Canada to the brink of qualification in the Davis Cup Finals group stage, comfortably dispatching Finland in Manchester.
Shapovalov needed just an hour and 13 minutes to beat Eero Vasa 7-6 (7-2) 6-2, backing up his win against Argentine Francisco Cerundolo on Tuesday.
The Canadian served five aces to his opponents' three, producing three love games in the final set to get his nation off to a flying start.
Auger-Aliassime followed up Shapovalov's victory with another straight sets win, this time over Otto Virtanen, emerging a 6-2 6-3 victor.
The world number 21 was dominant on serve throughout his 73-minute triumph, saving all three break points he faced and winning 86% of his first-serve points.
Auger-Aliassime and Shapovalov then prevailed in the doubles against Harri Heliovaara and Virtanen in another straight sets win, putting them top of Group D without dropping a set against their opponents.
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