Australian Open champion Aryna Sabalenka lost for the first time in 14 matches, and Coco Gauff set up a semi-final clash with Iga Swiatek at the Dubai Tennis Championships on Thursday.
Sabalenka fought back from a set down to end the title defence of Jelena Ostapenko a day earlier, but she came up short against Barbora Krejcikova as her Czech opponent overcame a tough first set to run out a 0-6 7-6 (7-2) 6-1 victor.
Sabalenka, playing in her first event since claiming a maiden grand slam title in Melbourne, romped into the lead by breaking Krejcikova's serve three times to go a set ahead.
But the world number two was pegged back in the second as Krejcikova battled to force a tie-break, which she dominated to level the match.
Krejcikova then completed the shock victory in style, breaking the second seed twice in the final set before clinching the win on her third match point to end Sabalenka's run of 13 straight triumphs.
Karolina Muchova's withdrawal due to an abdominal injury means it is Jessica Pegula who will face Krejcikova in the semi-finals on Friday.
After Swiatek reached the final four by virtue of Karolina Pliskova pulling out because of an illness, Gauff set up a sixth meeting with the world number one by beating Madison Keys 6-2 7-5.
In those five previous clashes between the pair, Gauff is yet to win a set.
But after cruising past Keys, doing so by converting four of five break points and winning 90 per cent of her service games, Gauff is confident of improving her dismal record against Swiatek on Friday.
"All five times, I did something wrong," Gauff said. "To be honest, she's playing great tennis and there's a reason she's world number one.
"Tomorrow I have no pressure. I just have to play my game. I definitely think I've gotten better since the last time I played her.
"Ranking is just a number at the end of the day. You just have to step on the court believing you can win, and that's what I'm going to do tomorrow."
World number one Swiatek sees off Xiyu in Madrid opener
World number one Iga Swiatek overcame Wang Xiyu with the minimum of fuss on Thursday to progress to the third round of the Madrid Open.
Swiatek has yet to win the title in Madrid, which is the only clay-court European event at which the Pole has not triumphed, but she got off to a flying start with a 6-1 6-4 victory over Xiyu.
"I love this place. I got to know the city a little better last year," Swiatek said. "So this time I feel more comfortable around."
Next up for the 22-year-old is a third-round tie against 27th seed Sorana Cirstea on Saturday.
Data Debrief
Swiatek has registered 150 match wins on the WTA Tour since the start of 2022. Elena Rybakina and Coco Gauff are the next best with 108 wins each.
She also became the first player born this century to claim 50 combined match wins in Madrid, Rome and Roland-Garros, since the inaugural women's singles event at the Madrid Open in 2009.
Dominant Gauff double bagels Rus at Madrid Open
Coco Gauff made a flying start to her Madrid Open campaign on Thursday, recording the first double bagel main-draw win of her career as she cruised past Arantxa Rus to reach the round of 32.
Gauff needed just 51 minutes to see off 33-year-old Rus as she booked a meeting with Ukraine's Dayana Yamstremska for Saturday.
The US Open champion lost just 18 points throughout the match – eight in the opener and 10 in the second set – as she put any doubts over her recent form to bed in emphatic fashion.
Gauff's power proved too much for Rus in the opener, the third seed winning 88 per cent of points behind her first serve and converting three of four break points to go a set up in just 23 minutes.
Rus brought up four break points midway through the second set, but when she failed to convert them, Gauff punished her in ruthless fashion by reeling off another three games then finishing things with a terrific forehand winner.
Speaking after her win, the American said: "I played really well today. It was just one of those days for me… 6-0 6-0, I've never done that before, I'll probably never do it again."
Data Debrief: Gauff matches Azarenka, Halep feat
Gauff is just the third player to register a 6-0 6-0 win in the women's singles draw at the Madrid Open, following in the footsteps of two former world number ones in Victoria Azarenka and Simona Halep.
The American, who only turned 20 last month, is also the youngest player to double bagel an opponent in any main-draw match on the WTA Tour since Iga Swiatek did so against Karolina Pliskova in the 2021 Italian Open final in Rome.
Nadal cruises past teen Blanch to tee up De Minaur rematch in Madrid
Darwin Blanch presented little challenge for Rafael Nadal, who dropped just one game in a convincing 6-1 6-0 triumph at the Madrid Open.
Nadal is feeling his way back to fitness, but 16-year-old qualifier Blanch offered little resistance to the 22-time major champion on Thursday.
Making his farewell appearance at the Madrid Open, where he has won five titles, Nadal needed just 64 minutes to progress to round two.
"I think today I played against an opponent with a great future in front [of him], but today, still making mistakes," said Nadal.
"I just tried to be there, be solid all the time without taking a lot of risks. It worked well. I'm happy to be through and I wish him all the very best for the future."
Nadal's reward is a rematch with Alex de Minaur, who he lost to last week in Barcelona.
Data Debrief
This game saw the largest age gap (21 years, 117 days) between two opponents in the history of ATP Masters 1000 events.
Nadal has now registered 57 wins at the Madrid Open, which is 20 clear of any other player.
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