Anett Kontaveit fought back to secure her place in the last 16 of the Tallinn Open in her native Estonia against a spirited Xiyu Wang.
The in-form Wang made life difficult for top seed Kontaveit initially, impressively taking the first set against the world number four.
Kontaveit then appeared to be cruising to victory when she took the second set 6-2 and found herself 5-1 up in the third, but Wang staged something of a revival.
Wang won four games on the trot to stun Kontaveit, only for the 21-year-old to lose her nerve as her opponent broke again and then served out the match 3-6 6-2 7-5, taking her through to face Tereza Martincova.
Beatriz Haddad Maia was also caused problems by a Chinese player, with Xinyu Wang taking the third seed to three sets.
Haddad Maia took the first set fairly comfortably but was blown away in the second before her composure returned in the decider, winning 6-3 1-6 6-4 to also progress to the last 16.
The other seed in action on Tuesday was Jil Teichmann, and she had no such issues seeing off Laura Siegemund 6-4 7-6 (9-7), although the day's most impressive win saw Donna Vekic thrash Maileen Nuudi 6-2 6-0.
At the Parma Ladies Open, second seed Martina Trevisan was stunned 7-5 6-0 by Sara Sorribes Tormo.
It was a bad day for seeds throughout the draw in Italy. Anna Bondar (fifth), Nuria Parrizas Diaz (seventh) and Lucia Bronzetti (eighth) all lost in straight sets.
Third favourite Irina-Camelia Begu did buck that trend, however. She benefited from Viktoriya Tomova retiring, although the Romanian was already a set and 5-1 up.
Auger-Aliassime and Shapovalov sweep aside Finland at Davis Cup
Felix Auger-Aliassime and Denis Shapovalov moved Canada to the brink of qualification in the Davis Cup Finals group stage, comfortably dispatching Finland in Manchester.
Shapovalov needed just an hour and 13 minutes to beat Eero Vasa 7-6 (7-2) 6-2, backing up his win against Argentine Francisco Cerundolo on Tuesday.
The Canadian served five aces to his opponents' three, producing three love games in the final set to get his nation off to a flying start.
Auger-Aliassime followed up Shapovalov's victory with another straight sets win, this time over Otto Virtanen, emerging a 6-2 6-3 victor.
The world number 21 was dominant on serve throughout his 73-minute triumph, saving all three break points he faced and winning 86% of his first-serve points.
Auger-Aliassime and Shapovalov then prevailed in the doubles against Harri Heliovaara and Virtanen in another straight sets win, putting them top of Group D without dropping a set against their opponents.
Nadal confirms Laver Cup withdrawal to help Team Europe 'deliver the win'
Rafael Nadal confirmed he will not participate in next month's Laver Cup, believing there are "other players who can help the team deliver the win" for Team Europe.
Nadal last played at the Paris Olympics alongside Carlos Alcaraz in the men's doubles, with the Spanish duo knocked out at the quarter-final stage at Roland Garros.
The 38-year-old also competed in the singles but was hampered by a thigh problem in Paris and suffered a second-round loss to eventual gold medallist Novak Djokovic.
Nadal participated in the inaugural edition of the Laver Cup seven years ago, defeating Jack Sock to help Team Europe claim a 15-9 victory.
It is yet to be confirmed who will replace the 22-time grand slam champion in Berlin next month as captain Bjorn Borg now goes in search of adding to his star-studded line-up.
Alexander Zverev, Alcaraz, Daniil Medvedev, Casper Ruud and Stefanos Tsitsipas have already been confirmed as the participants to face Team World in Berlin.
Nadal posted a statement on social media, saying that he was making the decision for the benefit of the team.
“I’m really disappointed to share that I won’t be able to compete at the Laver Cup in Berlin next week," Nadal said.
"This is a team competition and to really support Team Europe, I need to do what’s best for them and at this moment there are other players who can help the team deliver the win.
"I have so many great, emotional memories from playing in the Laver Cup, and I was really looking forward to being with my teammates and with Bjorn in his final year as Captain.
"I wish Team Europe the very best of luck and will be cheering them on from afar.”
The news will accelerate speculation that retirement could be imminent for the Spaniard, who was unsure of his future following his exit from the Olympic Games.
Alcaraz grinds through to help Spain to Davis Cup win
Carlos Alcaraz got a stroke of luck as he helped Spain make a winning start to the Davis Cup Finals group-stage campaign against Czechia as Tomas Machac retired through injury.
The French Open and Wimbledon champion dropped the first set 6-7 (3-7), but rallied to finish the second 6-1, forcing a decider after getting two vital breaks.
However, Machac pulled up in the opening game of the third set with cramp and was unable to carry on after one hour and 41 minutes on the court.
It followed Roberto Bautista's flying start in their opening singles match in Group B as he cruised past Jiri Lehecka 7-6 (7-1) 6-4, with the Czech making 27 unforced errors as he failed to find an edge.
In the doubles, Alcaraz and Marcel Granollers then had to dig deep, coming from behind to beat Adam Pavlasek and Jakub Mensik in three sets to give Spain a 3-0 victory and take them top of the group.
Meanwhile, Great Britain also made a bright start to their campaign in Group D, despite US Open semi-finalist Jack Draper sitting out.
Dan Evans got them started in Manchester with a hard-earned 7-6 (7-3) 6-2 victory over Eero Vasa of Finland, converting both of his break points in the second set.
Billy Harris followed that up with an impressive tournament debut, looking unflappable as he earned the hosts an unattainable lead with his 6-4 7-6 (7-4) win over Otto Virtanen.
Finland got themselves on the board in the doubles though, with Evans and Neal Skupski unable to get the better of Harri Heliovaara and Virtanen ensuring it was only a 2-1 loss.
Elsewhere, the United States joined Germany at the top of Group C with their 3-0 win over Chile and Italy's Matteo Arnaldi held off a spirited Thiago Monteiro to put them in control against Brazil in Group A after Matteo Berrettini's earlier victory.
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