Palmeiras midfielder Danilo has been granted his first call-up to the Brazil squad, while Tite selected Fabinho for the upcoming friendlies in June despite injury concerns over the Liverpool star.
Brazil announced a 27-man squad on Wednesday for friendlies against Korea Republic, Japan and South American rivals Argentina next month.
Fabinho is one of 13 Premier League players called up, despite the midfielder limping off in Liverpool's 2-1 win over Aston Villa on Tuesday after suffering a muscle injury.
Reports suggest Fabinho will miss the upcoming FA Cup final against Chelsea, along with league games against Wolves and Southampton, as he faces a race against the clock to feature in the Champions League final against Real Madrid on May 28 as Liverpool hunt an unprecedented quadruple.
Fellow midfielder Danilo was the other notable inclusion by Tite, with the Palmeiras man earning his maiden call-up after impressing at the Club World Cup, where his side were defeated in the final by Chelsea.
On Danilo's inclusion, Tite told reporters: "Danilo is one of those players that we have been following, we are always attentive to players that are emerging.
"Danilo played well at the Club World Cup in games that we followed live and made visits to the club. We were looking at a series of things on how he performs so we could justify his selection."
Fabinho's Liverpool team-mate Alisson also made the list, along with fellow goalkeeper Ederson, while Manchester United duo Alex Telles and Fred were named alongside Arsenal's Gabriel Magalhaes.
Newcastle United's Bruno Guimaraes was again selected by Tite and will look to add to his six caps, but there was no room for Magpies team-mate Joelinton, who has impressed since dropping into midfield under Eddie Howe.
Casemiro could partner Guimaraes in the middle alongside Lyon's Lucas Paqueta, while Weverton and Guilherme Arana were the only other two players to feature from Brazil's domestic league.
Tite will have vast experience in the backline to call upon as well, with the likes of Alex Sandro, Dani Alves, Thiago Silva and Marquinhos to select from.
Up top, Gabriel Jesus will look to carry over his scoring form from his exploits with Manchester City, with Neymar, Vinicius Junior and Real Madrid's Champions League hero Rodrygo part of a star-studded attacking line-up.
The upcoming friendlies are part of Brazil's penultimate preparations for the 2022 World Cup, where Tite's side are placed in Group G alongside Serbia, Switzerland and Cameroon.
Brazil squad: Alisson (Liverpool), Ederson (Manchester City), Weverton (Palmeiras); Alex Sandro (Juventus), Alex Telles (Manchester United), Dani Alves (Barcelona), Danilo (Juventus), Guilherme Arana (Atletico-MG), Eder Militao (Real Madrid), Gabriel Magalhaes (Arsenal), Marquinhos (Paris Saint-Germain) and Thiago Silva (Chelsea); Bruno Guimaraes (Newcastle United), Casemiro (Real Madrid), Danilo (Palmeiras), Fabinho (Liverpool), Fred (Manchester United), Lucas Paqueta (Lyon) and Philippe Coutinho (Aston Villa); Gabriel Jesus (Manchester City), Gabriel Martinelli (Arsenal), Matheus Cunha (Atletico Madrid), Neymar (Paris Saint-Germain), Raphinha (Leeds), Richarlison (Everton), Rodrygo (Real Madrid) and Vinicius Junior (Real Madrid).