Manchester City defender Ruben Dias has been left out of Portugal's squad for this month's crucial World Cup play-offs, as the 24-year-old continues his recovery from a hamstring injury.

The central defender has been out of action since sustaining the injury in the Premier League champions' FA Cup win over Peterborough United last month.

Fernando Santos will also be unable to call upon fellow injury absences Renato Sanches and Nelson Semedo, as he seeks to avoid being the first Portugal coach to fail to qualify for a major tournament since the country missed out on the 1998 World Cup in France.

After a last-gasp 2-1 loss to Serbia saw Portugal fail to qualify automatically for the World Cup, Portugal will host Turkey in a play-off semi-final on March 24, before the winner of that clash takes on either Italy or North Macedonia five days later for a place in Qatar.

Dias was named Premier League player of the season for the 2020-21 campaign, and has contributed to the league leaders keeping a joint-high 17 clean sheets in 29 top-flight games this term. 

In Dias' absence, uncapped Sporting CP defender Goncalo Inacio gets his second senior call-up, with 38-year-old Lille defender Jose Fonte and Porto's 39-year-old stalwart Pepe likely to form an experienced defensive partnership.

Cristiano Ronaldo, who has hit 115 goals in 184 appearances for his country's senior team, is one of three Manchester United players to make the squad, being joined by Diego Dalot and Bruno Fernandes.

Speaking to the press after announcing his squad, Santos emphasised the importance of the Turkey match, and insisted his 37-year-old captain remains a crucial part of his group.

"We have to win," said the Euro 2016 winner. "We have a goal, we have to fulfil it, it's a goal of the people. 

"All the national team's games will always be very important for the people. [We must] play for Portugal, as there have been others who have marked the history of Portuguese football.

"More important than anything is Portugal. That is the case for us and for the people that we are going to play for, and that also includes our captain Cristiano Ronaldo, who is a very important piece."

Although Dias will not play any part in the crucial match-up with Turkey, club team-mates Joao Cancelo and Bernardo Silva have both been named in Santos' squad.

Portugal squad: Anthony Lopes (Lyon), Diogo Costa (FC Porto), Rui Patricio (Roma); Cedric Soares (Arsenal), Joao Cancelo (Manchester City), Diogo Dalot (Manchester United), Goncalo Inacio (Sporting CP), Jose Fonte (Lille), Pepe (FC Porto), Nuno Mendes (Paris Saint-Germain), Raphael Guerreiro (Borussia Dortmund); Danilo Pereira (Paris Saint-Germain), Matheus Nunes (Sporting CP), William Carvalho (Real Betis), Ruben Neves (Wolves), Bruno Fernandes (Manchester United), Joao Moutinho (Wolves), Otavio Monteiro (FC Porto); Bernardo Silva (Manchester City), Joao Felix (Atletico Madrid), Diogo Jota (Liverpool), Goncalo Guedes (Valencia), Rafael Leao (Milan); Andre Silva (RB Leipzig), Cristiano Ronaldo (Manchester United).