Argentina left Juan Foyth and Lucas Ocampos out of their Copa America squad as Lionel Scaloni finalised his plans for the tournament in Brazil.
Scaloni made a last-minute adjustment as Bayer Leverkusen forward Lucas Alario was ruled out by injury and River Plate rising star Julian Alvarez took his place, .
The omissions of Foyth, who on Friday sealed a move from Tottenham to Villarreal, and Sevilla winger Ocampos, may be held up to scrutiny later.
However, Scaloni chose a 28-man squad packed with pedigree as Argentina attempt to conquer the Copa America for the first time since 1993, with Lionel Messi, Sergio Aguero and Lautaro Martinez giving the Albiceleste a formidable forward line.
Argentina face Chile, Uruguay, Paraguay and Bolivia in Group B of the South American championship. They were due to be co-hosts of the tournament but COVID-19 factors saw it move to Brazil at late notice.
Alario had been included on the initial final squad list sent to organisers CONMEBOL, but he was eventually excluded due to the hamstring injury that forced him to miss the closing weeks of Leverkusen's season.
Alvarez, 21, did not feature on Argentina's provisional list of 50 players but was allowed to join the squad because of Alario's injury.
CONMEBOL said in a statement: "This Friday, June 11, the Argentine Football Association (AFA) informed CONMEBOL of the injury of Lucas Alario, a player included in the list of 28 summoned for the CONMEBOL Copa America 2021 that had been delivered on Thursday, June 10.
"Given this, the Argentine Football Association requested the replacement of said player and the inclusion in his place of Julian Alvarez, a footballer not included in the first instance in the preliminary list presented on June 1.
"Said request was approved by the CONMEBOL medical commission in accordance with the provisions of the regulations and after the delivery of Form 2 [substitution of injured players]."
Argentina squad for Copa America:
Franco Armani (River Plate), Emiliano Martínez (Aston Villa), Agustin Marchesín (Porto), Juan Musso (Udinese); Gonzalo Montiel (River Plate), Nicolas Otamendi (Benfica), German Pezzella (Fiorentina), Nicolas Tagliafico (Ajax), Lucas Martínez Quarta (Fiorentina), Marcos Acuna (Sevilla), Lisandro Martínez (Ajax), Cristian Romero (Atalanta), Nahuel Molina Lucero (Udinese); Leandro Paredes (PSG), Guido Rodríguez (Real Betis), Giovani Lo Celso (Tottenham), Exequiel Palacios (Bayer Leverkusen), Angel Di María (PSG), Rodrigo De Paul (Udinese), Alejandro Gomez (Sevilla), Angel Correa (Atletico Madrid), Nicolas Dominguez (Bologna); Lionel Messi (Barcelona), Lautaro Martinez (Inter), Joaquin Correa (Lazio), Julian Alvarez (River Plate), Sergio Aguero (Barcelona), Nicolas Gonzalez (Stuttgart).