The former midfielder has been placed in charge of Spurs following the dismissal of initial caretaker boss Cristian Stellini, who was sacked following a 6-1 loss to Newcastle United.
Mason previously was in charge of Tottenham on a temporary basis in 2021 following Jose Mourinho's exit, and led them in their EFL Cup final loss to Manchester City.
Ahead of his first game at the helm again versus top-four rivals Manchester United, the ex-England international is looking for a response after being handed the reins again.
"My immediate focus is Thursday," he said. "The most important thing is a reaction. Sunday was obviously very disappointing for us.
"I would hope and expect there is a reaction from the players, from everyone. Another important thing is getting energy and positivity back into the group and the fans as well.
"We have to be realistic. With three training sessions before two games, it is hard to change a big deal, but I would hope before the end of season people would see my stamp on the group."
On returning to the top seat in the dugout for a second time, Mason gave assurances he feels up to the task at hand, adding: "It came about relatively easily from my point of view."
"Naturally I feel comfortable, and it is just part of being involved in football. From a personal perspective, we handled it well two years ago.
"Obviously a lot of things have happened [since then] but ultimately I feel ready."
Tottenham lie sixth in the Premier League with just six games to go, and face a battle to ensure their European qualification for next season.
They are six points behind fourth-place United, who have games in hand on them, though Spurs in turn have a match to spare compared with fifth-place Aston Villa.
Spurs found themselves three goals down in the first 15 minutes at Anfield as Curtis Jones, Luis Diaz and Mohamed Salah netted for Jurgen Klopp's men on Sunday.
Just a week on from a 6-1 defeat at Newcastle United, in which they were 5-0 down after 21 minutes, Tottenham this time almost pulled off an unlikely comeback.
Harry Kane and Son Heung-min set up a tantalising finish before Richarlison equalised in the 93rd minute, only for Diogo Jota to score a minute later to snatch a 4-3 win for Liverpool.
That saw the Reds leapfrog Spurs into fifth, and Kane acknowledged his team can have no complaints after another dire first-half showing.
"There's only so many words you can use, the bottom line is you need to go out there and show it isn't that case," the England captain told Sky Sports.
"It's on us to go away, find a reason why and see how it improves the situation. In these moments and these games, you can't do what we did at the end. It's a feeling of gut-wrenching defeat.
"The table doesn't lie, where we are doesn't lie. We've got some fantastic players and moments, but as a team we aren't playing good enough collectively.
"We need to show we can go through games when it isn't going our way. We deserve to be where we are.
"We have four games. This one is going to be hard to take, but we have a week until the next game, so we have to move on."
An insipid first-half showing from Tottenham was almost atoned for when Richarlison headed in, although the celebrations were soon cut short as Lucas Moura inadvertently rolled in Jota to slot past Fraser Forster.
Spurs were aggrieved substitute Jota was still on the pitch at that point, though, with claims the Portugal forward should have been dismissed for a second-half aerial lunge on Oliver Skipp.
Jota was shown a yellow card for catching the head of Skipp with a flying boot, although interim Tottenham boss Ryan Mason believed the tackle deserved a red.
Mason told BBC Radio 5 Live: "Honestly, it's probably the clearest red card you will see on the football pitch. I need an explanation.
"It endangered the opponent, Skipp needs stitches, and Jota has scored the goal when he shouldn't be on the pitch."
Former Spurs midfielder Mason took charge on Monday, with Stellini removed from his role following a dismal 6-1 defeat at Newcastle United – who led 5-0 within just 21 minutes.
While Tottenham appear highly unlikely to contend for a top-four finish, there are still European spots up for grabs as they prepare to host Erik ten Hag's Red Devils.
Asked what he expected to see from Spurs against United, Mason said: "A reaction, absolutely.
"Obviously Sunday was disappointing for us, and I would hope and expect there's a reaction from not only the players, but everyone in the building and everyone involved in the club.
"I've been in football long enough to know things can turn around very quickly. I believe we can make a positive impact. We have a game on Thursday to try and react and get back to winning ways.
"So the feeling and the thought isn't on the whole situation – it's about how we're going to influence the players and get some positivity back."
United approach Thursday's game in high spirits, having reached the FA Cup final through Sunday's penalty shoot-out win over Brighton and Hove Albion.
Possessing a six-point lead over Tottenham with two games in hand, United could effectively end their hosts' ailing top-four hopes with a victory.
Asked how important the game was to United's own bid for Champions League football, Ten Hag said: "Very. You see the table. It is quite clear.
"But our approach will not change. Our approach is we want to win every game.
"I have to make sure that our players are ready for tomorrow. I have to make sure that they have energy and are on the front foot to go into battle."
PLAYERS TO WATCH
Tottenham – Harry Kane
Kane has scored 99 Premier League home goals for Tottenham, and is bidding to become the first player to reach a century of goals in both home and away games in the competition.
He has scored five league goals against Manchester United in his career, but only one of those has come at home – in the final game played at White Hart Lane in May 2017.
Manchester United – Marcus Rashford
Having scored 10 goals in his first 10 Premier League appearances following the World Cup break, Rashford has now scored just once in his last five outings in the competition.
Just three of his 15 league goals this term have come away from home, but with the Red Devils harbouring doubts over Bruno Fernandes' fitness, Rashford will be required to step up in north London.
MATCH PREDICTION – TOTTENHAM WIN
United have won their last three Premier League games by an aggregate score of 5-0. They have not won four successive matches in the competition without conceding since their final season under Alex Ferguson in 2012-13, winning six consecutive games to nil between February and March 2013.
The Red Devils have won 39 Premier League games against Tottenham. No side has beaten a single opponent more often in the competition's history, while they are also unbeaten in seven midweek (Tuesday, Wednesday or Thursday) games in the Premier League this term (W5 D2).
However, the visitors have conceded 29 away goals in the Premier League this season, only ever shipping more on the road in a single campaign last term (35), and their injury problems may inspire hope of a new manager bounce for Spurs.
OPTA WIN PROBABILITY
Tottenham 42.0 per cent
Manchester United 30.1 per cent
Draw 27.9 per cent
