Union took an early lead after Lukas MacNaughton's 16th-minute own goal before Denmark international Uhre bagged a double before half-time.
Uhre added a third in the 56th minute to round out his hat-trick, tapping in Julian Carranza's low ball across goal and put the contest to bed.
Toronto pulled one back through Italy international Lorenzo Insigne in the 66th minute, before Richie Laryea's stoppage-time consolation.
New England reinforced their grip on top spot in the Eastern Conference with a 2-1 home win over Sporting KC, who are last overall.
Giacomo Vrioni netted a first-half brace for the Revs, but Sporting pulled one back in the 50th minute from Johnny Russell, only to be reduced to 10 men when Andreu Fontas was sent off 10 minutes later.
The Revs are ahead of second-placed Cincinnati on goal difference, with Cincy winning 2-1 over Portland Timbers after strikes from Sergio Santos and Brandon Vasquez.
New York City moved up to fourth in the East with a 3-1 home win over Dallas sparked by Santiago Rodriguez's double.
Western Conference leaders St Louis City were denied victory after Michael Barrios' 90th-minute equaliser as they drew 1-1 at Colorado Rapids.
St Louis remain top in the West but only on goal difference from Seattle Sounders who condemned Minnesota United to three straight losses with a 1-0 victory.
Denis Bouanga netted his seventh goal of the MLS season as LAFC drew 1-1 at Nashville, while LA Galaxy snapped their winless start to the campaign with a 2-0 home victory over Austin.
Inter Miami's losing run extended to six in a row, compounding the pressure on boss Phil Neville, as they went down 1-0 at Houston Dynamo.
Real Salt Lake beat San Jose Earthquakes 3-1, Charlotte defeated Columbus Crew 1-0, struggling Montreal edged New York Red Bulls 2-0 and Christian Benteke scored as DC United won 3-1 at Orlando City.
The best defence in the MLS had to chase the result this time after Gustavo Bou's well-taken opener for the Revolution in the 61st minute, with goals from Mikael Uhre and Daniel Gazdag handing the Union the three points.
The Union still created little from open play but they were gifted goals to turn the result around via the dead ball. Uhre equalized in the 75th minute, heading into what was effectively an open goal after Revolution keeper Djordje Petrovic failed to claimed the ball after coming off his line at a corner.
Gazdag then scored the eventual winner in the 79th minute, converting from the penalty spot after he was clumsily shoved by Henry Kessler.
Jim Curtin's side moved to 39 points, building a four-point lead from New York City, whose derby against New York RB on Sunday is one of two games they have in hand.
CF Montreal also moved to outright fourth in the East on Saturday, with Lukas McNaughton's own-goal handing them a 1-0 win over Toronto FC.
Chad Ahston momentarily remains in charge of D.C. United until Wayne's Rooney's work visa is finalized and Minnesota United took full advantage, earning a 2-0 win at home.
The Chicago Fire claimed an upset 1-0 win over the Seattle Sounders, while Emerson Rodriguez's 93rd-minute goal gave Inter Miami a 3-2 victory over Charlotte FC.
Gonzalo Higuain bagged his second goal of the week for Inter, scoring the equaliser after Yordy Reina's first-half brace gave Charlotte a 2-0 load.
In the Western Conference meanwhile, Austin FC claimed the Copa Tejas with a 1-1 draw against FC Dallas, but now only hold a two-point lead over Los Angeles FC, who have two games in hand.
The race for the last playoff spot in the conference tightened up, with the Colorado Rapids defeating Los Angeles Galaxy 2-0.
Along with the Sounders' loss, only three points now separate the seventh-placed Galaxy from the Rapids, moved to 11th with the win.
A Maximiliano Urruti double within the opening half-hour put Austin in a commanding position at the interval, but they struggled in the second half, giving up eight shots to three despite 62.3 per cent of possession.
Sebastian Driussi clinched the result for Josh Wolff's side in the 68th minute, though, getting on the end of a flick-on from Nick Lima's long-throw at the back post to head home.
Austin have now gone five games undefeated in the league, bouncing back from Wednesday's elimination at the hands of San Antonio FC in the US Open Cup.
They remain a point ahead of the Los Angeles Galaxy, who scraped a 1-0 win at home to Nashville via Dejan Joveljic's winner in the 86th minute. Real Salt Lake slipped up away to Portland Timbers, playing out a goalless draw.
There were also goalless draws between Sporting KC and Columbus Crew, and Colorado Rapids and Charlotte FC.
They were leapfrogged by FC Dallas, who claimed their fourth win of the season with a 2-1 win over Houston Dynamo.
They left it very late in the Texan derby after Sabastian Ferreira's opener for Dynamo in the 33rd minute, only equalising in the 87th minute through Tsiki Ntsabeleng.
Facundo Quignon claimed the three points for the Toros, lurking unmarked at the back post from a corner to score in the third minute of injury time.
Philadelphia Union remained top of the Eastern Conference despite a 1-1 draw at home to Montreal CF, giving the impetus to Orlando City and New York RB to make up ground on Sunday.
San Jose Earthquakes moved from the bottom of the Western Conference with a shock 4-3 win over the Seattle Sounders. Cristian Espinoza scored the winner for his hat-trick in the fourth minute of injury time via the penalty spot.
In Saturday's other results, Minnesota FC defeated Chicago Fire 3-0, while DC United claimed a 3-2 win over New England Revolution.
Carlos Vela scored a double, with both goals coming from the penalty spot, as LAFC secured only their second victory from their past eight games.
LAFC have a 15-1-0 record when scoring first this season although Houston leveled the game in the 24th minute from Corey Baird's classy goal after Vela netted an eighth-minute spotkick.
Vela restored LAFC's advantage from the spot on half-time after Fabrice Picault's handball was spotted by the VAR, with Ryan Hollingshead lifting home a third in the 52nd minute.
LAFC remain top of the Western Conference, nine points ahead of second-placed Austin, with two more matchdays until the postseason begins.
Gonzalo Higuain scored a 94th-minute winner to boost Inter Miami's playoffs hopes with a 3-2 road win over DC United, who had Christian Benteke net his first goal for the club.
Higuain struck the winner in the fourth of eight stoppage-time minutes, with a first-time strike from Deandre Yedlin's low cross from the right.
Benteke had opened the scoring in the 22nd minute with a back-post left-foot volley, before Leonard Campana's double for Inter.
Taxiarchis Fountas equalised in the 57th minute before Higuain's late winner, which means Inter are seventh in the Eastern Conference, moving above Columbus Crew, who conceded again late in a 1-1 draw with Portland Timbers on Sunday.
The Crew were denied all three points with Santiago Moreno lashing home a 95th-minute equaliser for the Timbers, after Kevin Molino's first-half strike.
Vela struck twice within six first-half minutes, first from the penalty spot before getting in behind the Colorado defence and calmly finishing past goalkeeper William Yarbrough.
The former Mexico international produced his best goal of the game to complete his hat-trick in the 50th minute, curling in a fine left-footed shot from just inside the box.
Vela only managed five goals from 20 MLS appearances last season with LAFC head coach Steve Cherundolo cautiously optimistic about his potential impact this term.
“We will have to wait and see what it does. But I think we all know what he can do,” Cherundolo said. “He showed it again today and he worked very hard in preseason to get to where he is today. I'm very happy for him and the group that we're all rewarded for it.”
Yimmi Chara's spectacular 78th-minute goal earned Portland Timbers a 2-2 draw with 2021 Supporters' Shield winners New England Revolution.
Portland, who reached the MLS Cup final last year, found a way back with Chara firing home a stunning overhead goal while Jozy Altidore debuted for the Revs as a 79th-minute substitute.
Ex-Liverpool and Bayern Munich midfielder Xherdan Shaqiri made his debut for Chicago Fire as they drew a blank in a 0-0 draw at Inter Miami.
Leonardo Campana's 10th-minute header from Gonzalo Higuain's cross hit the crossbar for Miami, while Chicago's Stanislav Ivanov struck the upright in the 31st minute. Shaqiri was impressive throughout, with a long-range first-half chance his only major goal attempt.
Toronto, who made a major off-season signing with Lorenzo Insigne to join in July, only managed a 1-1 draw at Dallas.
Philadelphia Union started its new season with a 1-1 home draw against Minnesota United, with Cory Burke levelling Robin Lod's 23rd-minute strike.
New York Red Bulls won 3-1 at San Jose Earthquakes, while Columbus Crew swept aside 10-man Vancouver Whitecaps 4-0.
Cecilio Dominguez netted a double as Austin won 5-0 over last year's bottom side Cincinnati, while Michael Estrada scored a brace as DC United eased past newcomers Charlotte 3-0.
Goals in the second half from Carlos Vela and Jose Cifuentes handed LAFC the three points, to put them three points clear of Austin FC, who have a game in hand.
James Igbekeme missed from close range for the Crew in the third minute and with the majority of possession, failed to create anything of substance from that point.
LAFC were able to punish them in transition through Vela in the 62nd minute, darting in behind the defensive line before finishing past Eloy Room.
Cifuentes doubled the margin in the 73rd minute, left with a relatively simple finish after Cristian Arango claimed the ball just outside the penalty area in a comical loss of possession from a Crew goal kick.
The win moves LAFC to 26 points from 13 games but elsewhere in the Western Conference, Nashville SC were unable to move to fourth, twice letting the lead slip in a 2-2 draw with Atlanta United.
In the Eastern Conference, DC United and Toronto FC also played out a 2-2 draw, while Tommy McNamara's spectacular finish in the 89th minute handed the New England Revolution a 3-2 win over Cincinnati.
The former England captain had been in charge at the Washington club since last summer but a 2-0 victory over New York City FC proved to be his final match.
CEO and co-chairman Jason Levien said on dcunited.com: “We have spoken with Wayne and agreed it is best for us to part ways at this time.
“This decision creates the avenue for our next general manager to have the full opportunity to impart a new philosophy and structure onto our sporting operations, which begins with the critical identification of a head coach who will best align with this.
“We are grateful to Wayne Rooney for all he has done for our club and for soccer in the nation’s capital, first as a DC United player and captain and most recently as our coach. He remains an important part of the DC United family and a valued and cherished friend.”
Rooney first moved to the MLS in 2018, spending 15 months in Washington as a player, and returned last summer after cutting his managerial teeth at Derby.
The 37-year-old improved DC United’s points tally but is heading back to England after their play-off hopes ended.
“It’s just the right time,” said Rooney in quotes reported by the Washington Post.
“I think I’ve done everything I can to try and get the club into (the) play-offs. It’s not one single thing which has happened, it’s about timing in your career. I have really enjoyed my time here. But I just feel it’s the right time to go back to England. What lies ahead, I don’t know.”
The head coach watched on in a baseball cap as Chris Durkin and Taxiarchis Fountas struck late on, stunning an Orlando side who had led since the ninth minute after Junior Urso's sublime chipped finish.
Durkin fired DC level from close to the penalty spot in the first added minute after the regulation 90 was complete, finding the top-left corner with a first-time finish after meeting Ola Kamara's clever low cutback from the left wing.
A draw would have been a decent result for DC, who sit at the foot of the Eastern Conference, but it got even better as a superb goal won it. A volleyed cross from the left by Kimarni Smith was met 12 yards out by Fountas, and he deftly diverted the ball into the bottom-right corner. It was a team-leading 11th goal of the season for Fountas.
It meant that DC United won an MLS game after trailing at half-time for the first time since March 7 2020, when they did so against Inter Miami. That dismal 25-game barren run is over, with the Rooney impact already being felt.
The record goalscorer for Manchester United and England has joined DC – a team where he also had a two-season playing stint – after recently leaving English club Derby County.
Midfielder Ravel Morrison, recruited by Rooney, attempted 96 passes in the game, and that ranks as the highest total for a DC United player in MLS this season.
Orlando had not lost an away game in MLS when leading at half-time since September 22, 2019, the date they allowed a lead to slip against Houston Dynamo.
