Chelsea boss Emma Hayes feels her side are fully prepared to kick off their FA Cup title defence without prolific striker Sam Kerr, who scored the winner in last year’s Wembley final.

The Australia captain on Thursday underwent surgery for an anterior cruciate ligament (ACL) injury and is set for a lengthy spell on the sidelines.

The Women’s Super League-leading Blues first have to find their way past West Ham, who have already been active in the transfer window with the signings of Shelina Zadorsky, Katrina Gorry and Kerr’s fiancee Kristie Mewis, while Chelsea have added defender Nathalie Bjorn from Everton.

Hayes, whose trophy cabinet already includes five FA Cups, said: “I know Sam would expect me to say nothing less. Our focus has got to be on the players that are fit and a good challenge for me is to find those solutions.

“I trust in the squad that we have and we’ve added to that as well.”

The competition’s overall prize pot has been doubled to almost £6million this season, with a minimum £54,000 for fourth-round winners while the losing clubs receive £13,000 – up from £15,000 and £3,750 respectively.

West Ham boss Rehanne Skinner said: “The cup is an incentive in itself but the prize money is a huge incentive, because it’s a significant amount of money when you’re talking about players and making additions.

“Increasing the prize money is really important in helping the growth of the game.”

Fifth-tier Luton are the lowest-ranked team remaining and will face WSL competition for the first time when they host Brighton.

Albion head coach Melissa Phillips said: “The FA Cup is such a special event, the history of the tournament, the magic as they say and what a fantastic match-up we have, a tier-five team and a top-tier team. They’ll certainly be up for the game.”

Phillips’ side were beaten in last year’s semi-final by Manchester United, who take on National League Northern Premier Division leaders Newcastle with over 1,000 Magpies fans preparing to make the trip to Leigh Sports Village.

United boss Marc Skinner confessed last year’s 1-0 loss in the final “feels like yesterday” and vowed his side “want to go one better than we did last year”.

Newcastle striker Katie Barker insisted her side are brimming with confidence, saying: “No matter who we play, we always play a game to win and we’ll do everything we can to win the game.”

Arsenal boss Jonas Eidevall vowed to take the competition seriously, beginning against Watford. He said: “There’s no excuse for not being able to field your best teams in these competitions.”

Durham’s meeting with Manchester City, selected for broadcast on the BBC, will mark the first time the Championship side have been televised.

Durham midfielder Lauren Briggs told the club website: “It’s something we all dream of, a great opportunity, and we’ve worked really hard for this.”

Bristol City host Liverpool in an all-WSL match-up while Championship Sheffield United travel to Tottenham and tier-three Derby welcome top-flight Leicester.

Sunderland face Southampton, Blackburn make the trip to Crystal Palace, Plymouth face Nottingham Forest and Ipswich meet Charlton.

Tier-three Burnley play Championship leaders Birmingham, Reading take on Wolves and London City Lionesses play Moneyfields.

Tottenham captain Shelina Zadorsky is relishing their “must-win” showdown against fellow Women’s Super League strugglers Reading on Saturday.

Both sides will be desperate for victory in their fight to avoid relegation, with 10th-placed Spurs three points above rock-bottom Reading heading their penultimate game of the season.

Vicky Jepson’s Tottenham have hit a rough patch, picking up only two points from their last five games, while Reading have lost five out of six and were thrashed 5-0 at home by Aston Villa in their last outing.

The match, which is part of a double-header at the Tottenham Hotspur Stadium, with the men’s team kicking off against Brentford at 1230pm in the Premier League before the WSL fixture takes place at 1615pm, could have huge implications for both clubs, but Zadorsky is looking forward to the occasion.

The Canada international told the club website: “We’re all excited, this is a must-win game for us and we’re very cognisant of where we are in the table and taking accountability for that as players.

“But in training there has been good energy and we’re really pushing to get a good result in front of our fans at home.

“We know how important the game is. We need to enjoy it and find a way to win.”

Saturday’s fixture is also huge for a Reading side whose last win was against West Ham at the start of March.

A victory against Tottenham would see the Royals move off the bottom if Leicester lose to West Ham on Sunday, and manager Kelly Chambers knows what is at stake for her team.

She told the club website: “It’s a hard moment, as I see it every day the amount of hard work that goes in from my staff, the players are given me everything during the week, we’re all fighting.

“I think if we’re all brutally honest, many would have predicted us to be in the position we are in, some have said it from the moment we were promoted, but time and time again we’ve proved what we’re about and on Saturday we need to do it again.

“This Saturday there will be no place to hide.”