Frustrated Gregor Townsend refused to entertain questions about his future after Scotland suffered a calamitous second-half collapse on their way to a costly 31-29 Guinness Six Nations defeat in Italy.

The Scots arrived in Rome aiming to enhance their bid for a first top-two finish in the championship this century, while they still held out some hope of winning the title. However, their loss in the Eternal City, allied to England’s win over Ireland, leaves them staring at the prospect of a bottom-half finish.

Things looked to be going well for Townsend’s team when they led 14-3 and 22-10 during the first half following tries by props Zander Fagerson and Pierre Schoeman and wing Kyle Steyn. A disallowed George Horne try early in the second half following a foul by Schoeman proved pivotal though.

Italy, who had scored in the first half through Martin Page-Relo, turned the match in their favour with tries from debutant Louis Lynagh and substitute Stephen Varney. And some excellent kicking under pressure from Paolo Garbisi took the game away from the Scots before Sam Skinner’s late try gave them a glimmer of hope.

Scotland incredibly still have an outside chance of finishing in the top two as they prepare to head to Ireland for their final match next weekend, but losing to Italy for the first time since 2015 represents a major setback for Townsend just five months after an underwhelming display at the World Cup brought pool-stage elimination.

When the head coach, who is contracted until 2026, was asked if he felt his job was under threat after his side became the first to lose a Six Nations match in Rome since 2013, he said: “I’m not going to answer that question.

“We’re disappointed with the result today, but we know this team have come on since the World Cup.

“We can look at this result and be really disappointed, but I’ve been really proud of how this team’s played during the championship so far.

“I believe in this group. You can look at results and say: ‘We’ve lost to Italy, this team aren’t going to take that next step’. Or you can look at where we’ve grown since the World Cup.

“I still believe the performances the players are putting in can show we can beat anybody.

“But if we take our eye off the ball for a period of time, we can be beaten. That’s what happened today.”

Townsend admitted Scotland were architects of their own downfall – although he did acknowledge a superb performance from Italy, who won their first championship match since their victory in Wales two years ago.

“The credit has to go to Italy as well,” said Townsend. “There are two teams that play the game, not just one. I thought that Italy were very good even in the first half where we dominated most of the possession.

“But our actions after the try that was disallowed weren’t good enough. We gave Italy a way into the game through penalties and field position. If you give that to any team in the Six Nations, they’re going to take the points, which they did.

“It’s very frustrating that we didn’t get that try, which is down to our execution, and after that it was even more frustrating.”

Townsend admitted the result “hurts a lot” but he remains steadfast in his belief that Scotland are continuing to make progress overall under his stewardship.

“We won in Wales for the first time since 2002 this year,” he pointed out. “We retained the Calcutta Cup. We had a decision (over what would have been a match-winning try) go against us in the game with France.

“This result is very disappointing. We’ve lost against a good Italy side, one of the best Italian teams I’ve seen. But today is not what defines this team.”

Scotland are hopeful that talismanic wing Darcy Graham will be fit enough to play a part in the Guinness Six Nations even though he will stay on the sidelines for Saturday’s Calcutta Cup showdown with England.

The 26-year-old – who is second on the national team’s all-time try-scoring list – missed the opening two matches of the championship against Wales and France with a quad problem.

Graham was scheduled to return to the fold for this weekend’s Murrayfield clash with England, but it emerged on Monday that he had suffered a new groin issue while training with Edinburgh last week, which has ruled him out of contention.

The free-scoring back – who missed the entirety of last year’s Six Nations with a knee injury – is due to be assessed this week and Scotland are hoping he will be able to return for the championship-concluding fixtures away to Italy and Ireland next month.

“Darcy had started back with Edinburgh and we were very happy about that,” said scrum coach Pieter De Villiers at a media briefing on Tuesday.

“But he’s picked up a bit of a groin niggle at the moment so he’s just nursing that. We’ll see how that goes.”

Asked if Graham was expected back before the end of the tournament, De Villiers said: “We’ve got another fallow week (after England) so that will hopefully give him enough time and then we’ll just assess things from there.”

Despite Graham’s ongoing absence, Scotland will welcome back two senior members of their back three this weekend, with Glasgow wing Kyle Steyn returning after missing the France game for the birth of his child and Toulouse full-back Blair Kinghorn available after sitting out the first two games with a knee injury.

“Blair has been training really well and we’re excited to see him back in the squad again,” reported De Villiers.

Saturday’s Calcutta Cup clash will be Scotland’s first outing since their agonising defeat at home to France, when the officials controversially decided not to award them what would have been a match-winning try after Sam Skinner appeared to ground the ball on the whitewash in the last action of a tightly-contested affair.

De Villiers admitted that having last weekend off was beneficial in helping the Scots banish any lingering frustration at having their hopes of a Grand Slam dashed in such galling fashion.

“Yes, it almost feels long ago,” he said of the extra time out of camp to regroup. “A bit of time off was good for the team.

“There’s obviously been frustration and disappointment. I thought we’d done enough to win that game but that’s the way rugby goes. Sometimes things don’t go your way.

“It’s the best thing for us to move on and prepare for England which is a fantastic game to look forward to. It’s all behind us.”

De Villiers has no concerns about any hangover from the France game plaguing Scotland this weekend as he feels recovering from such setbacks is “part of what we do”.

“It’s important to be able to move on,” he said. “It’s important to be able to be frustrated as well.

“As much as there were things we could have done better, it’s good to know that we did enough (to potentially win the game) as well.

“That’s important for our psyche and for moving on. It’s not the first decision to go against us and it won’t be the last decision to go against us.”