Pau Cubarsi, Marcos Llorente and Aleix Garcia have been cut from Luis de la Fuente Castillo's Spain squad for Euro 2024.
De la Fuente named a 29-man party last month, with a youthful look to it, as Spain aim to regain European glory in Germany.
Cubarsi started Spain's friendly win over Andorra, setting up Ayoze Perez, who is a surprise inclusion in the final squad, for his debut goal.
Despite impressing in his breakout season in LaLiga, there is no place for the 17-year-old as De la Fuente opts for a more experienced group of centre-backs in Robin Le Normand, Nacho, Aymeric Laporte and Dani Vivian.
Llorente also misses out, with Cubarsi's fellow Barcelona youngster, Fermin Lopez, keeping his place after impressing with an assist in his first international appearance on Wednesday.
Garcia is the other midfielder not to make the cut despite drawing attention with Girona as they finished third in LaLiga, scoring three goals and adding six assists in the league in 2023-24.
Spain will begin their Euro 2024 campaign against Croatia in Group B on June 15, before playing Italy and Albania in their other group matches.
Final Spain squad: Unai Simon (Athletic Bilbao), Alex Remiro (Real Sociedad), David Raya (Arsenal), Dani Carvajal (Real Madrid), Jesus Navas (Sevilla), Aymeric Laporte (Al-Nassr), Nacho (Real Madrid), Robin Le Normand (Real Sociedad), Dani Vivian (Athletic Bilbao), Alex Grimaldo (Bayer Leverkusen), Marc Cucurella (Chelsea), Rodrigo (Manchester City), Martin Zubimendi (Real Sociedad), Fabian Ruiz (Paris Saint-Germain), Mikel Merino (Real Sociedad), Pedri (Barcelona), Alex Baena (Villarreal), Fermin Lopez (Barcelona), Alvaro Morata (Atletico Madrid), Joselu (Real Madrid), Dani Olmo (RB Leipzig), Nico Williams (Athletic Bilbao), Mikel Oyarzabal (Real Sociedad), Ayoze Perez (Real Betis), Ferran Torres (Barcelona), Lamine Yamal (Barcelona).