Bayern Munich head coach Thomas Tuchel urged his players to retain a positive mindset ahead of a crucial run of fixtures in the Bundesliga and Champions League.

Following a shock 2-1 defeat at home to Freiburg in the DFB-Pokal on Tuesday, Bayern travel to face the same opponents in the league on Saturday before going up against Manchester City in the first leg of their Champions League quarter-final next Tuesday.

Speaking at a press conference on Friday, Tuchel repeatedly mentioned a need to be "positive" despite the cup setback.

"We need to continue with a positive approach, we can be angry but still be positive," he said, later adding: "We need a reaction, but we would have needed one even if we won.

"People don't want to hear it but there were positive things in [the Freiburg] game... Things can happen where the result is probably not right after a game like this but we have to accept it.

"We are lacking a bit of precision in the last pass, a bit of trust, cohesion, creativity. We need form and that will only come back if we remain positive, even though we were unhappy with the result."

On the reaction of his players following the loss to a 95th-minute Lucas Holer penalty at the Allianz Arena, Tuchel added: "They are very critical of themselves. I was telling them the same thing I have told you, that we just have to accept it, that the coach is talking about positive things about the game even though no-one wants to talk about it.

"We can have a bad mood for a couple of days but that doesn't help anyone, you need a positive mindset to move forward."

Tuchel confirmed the Freiburg game will come too early for injured pair Eric Maxim Choupo-Moting and Mathys Tel, while Dayot Upamecano misses out through suspension.

When asked who can play as the striker in Choupo-Moting's absence, he said: "Mathys Tel can play as a nine, that was his answer when I asked him his position, but he is injured.

"Sadio Mane and Serge Gnabry can play there as well, they will be my players of choice."

The former Borussia Dortmund and Chelsea boss was not keen to look beyond Saturday to Tuesday's trip to Manchester, but he acknowledged he cannot use Freiburg as preparation given the difference in approach.

"I don't believe that we can practice the things we need against Man City, Freiburg play a completely different way," he said.

Thomas Muller was left feeling bitter after Bayern Munich were saddled "with the shambles" of another DFB-Pokal failure against Freiburg.

The Bundesliga leaders slipped to a 2-1 defeat in the quarter-finals, after Lucas Holer's 95th-minute penalty condemned them to a late loss.

Dayot Upamecano had handed Bayern the lead in Thomas Tuchel's second game in charge, before Nicolas Hofler squared matters in the first half.

It means the club will miss out on the DFB-Pokal final for the third straight season, and Muller concurred the loss represented a blow to their pride.

"[There is] a certain bitterness," he told Sky Sport Germany. "We're eliminated. You can't make amends anymore. You're standing there with the shambles.

"It's over again. Of course that scratches your sense of honour. Freiburg hardly had a chance to score, but if you lose in the end, you have to face the questions."

The result means it is one win and one loss for Tuchel since he succeeded Julian Nagelsmann, having picked up a 4-2 win over Borussia Dortmund in his first game.

Muller appreciated the need to adapt to their new coach, but still stressed the blow of their exit would linger, particularly with a rematch in the Bundesliga on Saturday.

"We [have] now had two games with a new coaching line-up," he added. "Now, we're emotionally down to earth. We have to manage that as a group.

"We will get back together on Thursday, we will try to improve. But now, there is disillusionment and brutal disappointment."

Thomas Tuchel accepted responsibility for Bayern Munich's DFB-Pokal quarter-final exit, after they suffered a late 2-1 loss against Freiburg.

The Bundesliga leaders looked set for extra-time at Allianz Arena, after Dayot Upamecano's header was cancelled out by Nicolas Hofler.

But Jamal Muisala's penalty concession deep into second-half stoppage time allowed Lucas Holer to seal a last-gasp victory for the visitors from the spot.

In only his second game in charge since replacing Julian Nagelsmann, Tuchel acknowledged he had to shoulder the blame for their defeat.

"I am not really angry," he said. "We conceded two goals with two shots. I can’t remember another chance from Freiburg.

"The last pass, [and the] precision obviously, are all an issue. But still, we had good moments we could have used, but [we] just could not keep it up.

"In the end, it is our fault. We are very disappointed [to lose] a quarter-final at home. Of course, I am responsible."

Tuchel made a winning start to his Bayern career with a decisive 4-2 win over old club Borussia Dortmund, where he previously won the DFB-Pokal.

His failure to back it up with progress to the semi-finals of Germany's major knockout competition will take the shine off his early impression.

Bayern can claim immediate revenge on Saturday when they travel to Freiburg for a reverse fixture in the Bundesliga, looking to consolidate top spot.

They also remain in the mix for Champions League honours, and return to European competition against Manchester City next Tuesday.

Lucas Holer's last-gasp penalty saw Freiburg snatch a dramatic 2-1 victory against Bayern Munich in the DFB-Pokal quarter-finals to end Thomas Tuchel's hopes of a domestic double.

The tie appeared to be headed to extra time, until Jamal Musiala handled Nicolas Hofler's strike, enabling Holer to secure a shock win.

Bayern had looked the likelier of the two teams to claim a late winner, after seeing Dayot Upamecano's first-half header cancelled out by Hofler's excellent volley.

It means Tuchel, in his second game since replacing Julian Nagelsmann, will miss out of the chance of both Bundesliga and Pokal glory as Christian Streich's side proceed to the last four.

Massimiliano Allegri was critical of Juventus' second-half performance after they reached the Europa League quarter-finals with a 2-0 win at Freiburg on Thursday.

Juve built on their 1-0 first-leg advantage as Dusan Vlahovic converted a first-half penalty after home defender Manuel Gulde was controversially sent off for handball inside his own area.

Federico Chiesa added a late second after entering the fray as a substitute, as Juventus maintained their bid for a first major continental title since they won the Champions League in 1996. 

However, Allegri was displeased with Juventus' performance after Freiburg went down to 10 men, saying the Bianconeri must improve if they are to progress further in the competition.  

"The team got a good result as we progressed. We made a lot of mistakes in the second half with our ball handling, and we need to improve," he said at his post-match press conference.

"We can't play a second half like this against a team of 10. We were made to defend our own box. The second half should have been better. We need to work; we made a lot of wrong decisions.

"Against 10 we lowered our attention and pace. The result is good – excellent even – but let's not get excited because we're in the quarter-finals. We have to think about what we did in the second half and improve."

Chiesa's stoppage-time goal – a driven strike which found the net via the foot of the post – was just his second since returning from an anterior cruciate ligament injury in November, and the Italy international is looking to find consistency in the coming weeks.

"My first thought is to be available to the coach. Unfortunately I haven't been there in the last few games," he said. "There's still a bit of discomfort, but I'm working even harder to be more ready.

"Unfortunately my father [former Italy striker Enrico Chiesa] had similar injuries. It's a path of great obstacles to come back, but even after that, the journey continues. I just have to stay calm."

Meanwhile, the decision to send Gulde off after his outstretched arm was struck by Federico Gatti's shot infuriated Freiburg boss Christian Streich, as did a failure to punish Wojciech Szczesny for seemingly picking up a Manuel Locatelli back-pass.

"We were brave, we went for it," the Freiburg boss said. "Then there was the situation with the red card and the penalty. That was of course a difficult situation for us.

"I'm speechless. In both games, after every foul, a Juve player plays the ball away. Always. It's a complete mystery to me. 

"Then there's the intentional back-pass that isn't blown. It's difficult to accept. Something that was crucial was let go."

Dusan Vlahovic and Federico Chiesa scored as Juventus saw off 10-man Freiburg to reach the Europa League quarter-finals, winning 2-0 in Germany to claim a 3-0 aggregate triumph.

Having established a slender lead in the Turin first leg, Juventus saw a Vlahovic effort disallowed for offside in Thursday's rematch before Manuel Gulde was dismissed for blocking a shot with an outstretched arm.

Vlahovic stepped up to convert the subsequent 45th-minute penalty and ease any Juventus nerves, before Chiesa came off the bench to add gloss to the scoreline late on.

Juventus were ultimately comfortable as they booked a place in Friday's last-eight draw, keeping hopes alive of European silverware – and qualification for next season's Champions League.

Freiburg penned back Juventus early on as they looked to level the tie, with Matthias Ginter forcing Wojciech Szczesny into a flying save with a downward header 22 minutes in.

The visitors thought they had the lead five minutes later as Vlahovic volleyed in after Gleison Bremer's header rattled the crossbar, but the Serbian's celebrations were cut short when a VAR review ruled him offside.

Juventus had better fortune with another VAR review as half-time approached. Federico Gatti's goal-bound shot struck the arm of Gulde as he attempted to block, earning the defender a second yellow card and allowing Vlahovic to score from the spot.

Despite their numerical disadvantage, Freiburg continued to press after half-time, Michael Gregoritsch firing a free-kick wide before Szczesny turned Lucas Holer's volley away.

Chiesa saw a stoppage-time effort tipped onto the crossbar by Mark Flekken as Freiburg tired, and the substitute had his goal less than a minute later as he drilled a low effort in off the left post.

Angel Di Maria and Federico Chiesa remain in contention to play in Juventus' Europa League last-16 second leg against SC Freiburg despite injury worries.

Di Maria appeared hampered by a left thigh injury late in the 1-0 first-leg win last Thursday, where he earlier netted the winner, and subsequently missed Juventus' 4-2 Serie A win over Sampdoria on Sunday.

Chiesa came on in the 67th minute against Freiburg but sustained a right knee injury, although the club confirmed it was not ligament capsule damage.

Bianconeri head coach Massimiliano Allegri hopeful that the pair would play some part in the second leg, although he conceded they would not be fit to start.

"It is very unlikely they will be there from the start, as we only recovered them this morning, but they could be of use during the match," Allegri told reporters.

"I will evaluate Chiesa and Di Maria tomorrow morning and from there I can make my decision on the starting line-up.

"Unfortunately, Di Maria had this injury after the first leg with Freiburg and had to miss quite a few training sessions.

"The test went well today, that doesn’t mean he has a lot of minutes in his legs, but there is the risk we could lose him for 40 days, seeing as that is what happened in September.

"Tomorrow is important, but we also have many more matches to play this season."

Di Maria has scored eight goals in all competitions with four Serie Aa assists this term, while Chiesa has contributed three league assists with one goal.

Allegri declared striker Dusan Vlahovic was fully fit and available to play although he declined to commit to starting him alongside Moise Kean.

Vlahovic has not scored in his past six appearances dating back to his goal against Nantes in the Europa League on February 16.

"Dusan Vlahovic was very sad that he hasn't scored recently, but is fine physically. I am relaxed and certain that he’ll soon be back on the scoresheet," Allegri added.

"We want to score here, because just trying to defend for 0-0 is really not a good idea. Freiburg are good on set plays, we need to be very organised and concentrated for the full 100 minutes."

Manuel Locatelli saluted "phenomenon" Angel Di Maria, stating his Juventus team-mate is "in another category" after their narrow win over Freiburg.

Di Maria scored the only goal as the Bianconeri drew first blood in the opening leg of the Europa League last-16 tie, thumping home a header from Filip Kostic's 53rd-minute cross.

Following his hat-trick against Nantes in the previous round, the World Cup winner has now been directly involved in 10 goals in 2023 (scored seven, assisted three).

The 35-year-old revealed he is in talks with Juve over extending his 12-month contract, which expires at the end of the season, and Locatelli hailed his team-mate's influence on the side.

"Angel is a phenomenon, he's in another category, I think he's really a champion," the midfielder said. "For us, it's an honour to have him with us in training and in the game. We have to be thankful that he is here.

"We are only 1-0 up, today was only the first half, we must play a mature game there [in Freiburg next week]. The 1-0 may be enough, but we have to go there and play a great game.

Although they lead, Juventus will feel their advantage should be greater, having converted just one of their 20 shots on goal during the first leg.

Head coach Massimiliano Allegri has urged his players to demonstrate a greater clinical edge, though he also encouraged Dusan Vlahovic to stay patient with the striker now having gone five matches without finding the net.

"We have to improve in the number of goals scored," Allegri said. "We create enough, the [conversion] percentage is low. We need lucidity when we attack the area, we have to improve.

"I'm happy because physically Dusan is much better, he has to be calm. Maybe on Sunday [against Sampdoria], he will score."

Angel Di Maria revealed he is in talks with Juventus over a new contract after his header saw off Freiburg in the first leg of their Europa League last-16 tie.

The Argentina international settled the opening leg at the Allianz Stadium when he powered home Filip Kostic's 53rd-minute cross for his fourth goal in this season's competition.

Di Maria, who also netted a hat-trick against Nantes in the previous round, has now been directly involved in seven goals in his last six European appearances.

The 35-year-old endured an injury-hit start to life in Turin after signing a 12-month deal last July, but has already registered 10 goal involvements in 2023 (seven goals, three assists).

Bruno Fernandes and Toulouse's Branco van den Boomen are the only other midfielders in Europe's top five leagues to hit double figures in the calendar year.

Di Maria's future is uncertain, but he said negotiations to extend his stint with Juve are ongoing.

"I'm happy to have helped the team, we are doing a good job and we must continue like this," he told Sky. "The important thing was to win, and we did it.

"We will do our best [to reach the final of this competition]. We will try to win both the Coppa Italia and the Europa League.

"We're talking, I'm happy here, and I'm finally feeling the affection of the people after a difficult start."

Allegri also hailed Di Maria's impact, while saluting his side's display ahead of the second leg in Germany next week.

"Tonight, [Di Maria] scored a wonderful goal but, above all, he gave an extraordinary performance.

"We played a very good match tonight, with great intensity and good technically. The only thing we missed too many times was the last pass. We could have chosen better and that made the difference.

"In Freiburg, it won't be easy, but the thing that makes me think positive is that they will give us spaces, and we will have to exploit them."

Angel Di Maria scored the only goal of the game as Juventus beat Freiburg in the first leg of their Europa League last-16 encounter.

Juve registered a whopping 20 shots at the Allianz Stadium, where Di Maria's second-half header Bianconeri gave them a slender lead in the tie.

The Argentina international continued to shine in this competition, having netted a hat-trick against Nantes in the previous round.

Freiburg, who saw their five-match unbeaten streak halted, must come from behind when the teams recommence battle in Germany next week.

Massimiliano Allegri's decision to drop Paul Pogba from the squad for disciplinary reasons dominated the build-up to what was Juve's first Europa League showdown against German opposition since they faced Borussia Dortmund in the 1994-95 semi-finals.

Pogba's fellow France international Adrien Rabiot almost opened the scoring when he drew smart reflexes out of Mark Flekken, who also kept out Juan Cuadrado's free-kick.

Bremer then somehow failed to find an open goal in the 31st minute, the defender heading over Di Maria's deep corner from just two yards out, while Cuadrado stung Flekken's palms once more before the break.

Juve eventually made the breakthrough eight minutes into the second half, when Di Maria powered home a header from Filip Kostic's centre.

Freiburg thought they had responded in the 62nd minute but, following a consultation with the VAR, Lucas Holer's wonderful volley was ruled out as Matthias Ginter handled in the build-up.

Vincenzo Grifo was then marginally off target with a free-kick that represented the visitors' only recorded attempt on goal, as the Serie A side saw out a narrow victory.

Paul Pogba has been dropped by Juventus for Thursday's Europa League clash with Freiburg in Turin.

The France international was reportedly late for a team meeting on Wednesday and was not included in Massimiliano Allegri's squad when it was circulated on the morning of the last-16, first-leg tie.

Pogba has endured a miserable start to his second spell with Juve after re-joining the club from Manchester United last July.

The 29-year-old midfielder has suffered with multiple injuries and had to wait until February 28 to make his second debut, coming off the bench in the 4-2 derby win against Torino and also appeared as a late substitute in Sunday's 1-0 defeat at Roma.

Thursday's game against Bundesliga club Freiburg had been earmarked as one in which he could make a long-awaited start with Allegri outlining his optimism at the pre-match press conference.

However, widespread reports in Italy have suggested Pogba was late to a team meeting 24 hours before the game with Allegri subsequently opting to leave the player out completely.

Paul Pogba's fitness will be assessed ahead of Juventus' Europa League meeting with Freiburg on Thursday, with the midfielder still eyeing his first start since his Bianconeri return.

Pogba made his long-awaited second Juve debut in a 4-2 win over cross-city rivals Torino last month after enduring several injury setbacks since re-joining from Manchester United last July.

Having appeared from the bench in that fixture, the France international did likewise in Sunday's demoralising 1-0 defeat at Roma in Serie A.

Asked if Pogba could make his first start of the season when Juventus host Freiburg in the first leg of their last-16 tie, boss Massimiliano Allegri was hopeful but uncertain.

Allegri said: "Yesterday [Tuesday] he did a good workout, today [Wednesday] he was separate from the group. Tomorrow [Thursday] morning we'll understand if he can be available.

"He didn't work with the team [on Wednesday]. I think he will be available, but we'll see."

An Angel Di Maria hat-trick fired Juve past Nantes in the last round of the competition, delivering a 3-0 away win after the Bianconeri toiled to a 1-1 draw at home to the French side.  

With Freiburg one of just three teams to go unbeaten in the Europa League's group stage this term (also Real Betis and Fenerbahce), Allegri is aware of the need for an improved home display.

"We want to lay the foundations for the second leg," he said. "They are physically strong, they are fifth in Germany, they are unbeaten in Europe. 

"We have to go back to winning at home – in the Champions League and in the Europa League we only got one win [at the Allianz Stadium this season].

"So we'll need attention, otherwise we'll have to do the same as against Nantes. We have to win on Thursday, whether it's by one or two goals – it won't be easy, it will be balanced."

Juventus will be able to draw on the experience of Filip Kostic, a Europa League winner with Eintracht Frankfurt last season, and the Serbian says lifting Europe's secondary club trophy is now the team's foremost aim.

"I know the competition, I won it with Eintracht – it was our dream to win the Europa League. With Juventus it's different, the goals were different, but now we want to win it," Kostic said.

"They are a tough, compact, solid opponent. To win this Europa League – which is our main objective – we will have to overcome them. We want to get as far as possible and we go game by game."

Manchester United have been drawn to face Real Betis in the Europa League's round of 16, while Arsenal will battle Sporting CP for a quarter-final spot.

United came from behind to clinch a 2-1 victory over Barcelona at Old Trafford on Thursday, and their reward for a 4-3 aggregate triumph over the Blaugrana is a tie against another Spanish opponent.

Having not lifted any silverware since winning this competition in 2017, United are in the hunt for four trophies this term, though Erik ten Hag's men will be tested by a side sat fifth in LaLiga.

Betis boss Manuel Pellegrini has faced United on four previous occasions in European competitions, with all four games finishing goalless during his time with Villarreal (in the 2005-06 and 2008-09 Champions League group stages).

Premier League leaders Arsenal will face Sporting after Ruben Amorim's team thrashed Midtjylland 5-1 on aggregate in the knockout round play-offs.

Sporting have progressed from each of their previous six ties against English teams in the knockout stages of the UEFA Cup/Europa League, with all six coming against different teams.

The other standout last-16 ties see Juventus meet Freiburg, while Jose Mourinho's Roma will take on Real Sociedad as they bid to follow up last season's inaugural Europa Conference League triumph.  

Feyenoord, who Roma beat in the final of that competition, have been paired with Shakhtar Donetsk, while surprise Bundesliga challengers Union Berlin will face Belgium's Union Saint-Gilloise.

Six-time winners Sevilla – the most successful club in UEFA Cup/Europa League history – will face Fenerbahce, while Bayer Leverkusen will take on Ferencvaros. 

The first legs of the last-16 ties will take place on March 9, with the group-stage winners hosting the return fixtures one week later. 

Europa League last-16 draw in full

Bayer Leverkusen v Ferencvaros
Juventus v Freiburg
Manchester United v Real Betis
Roma v Real Sociedad
Sevilla v Fenerbahce
Shakhtar Donetsk v Feyenoord
Sporting CP v Arsenal
Union Berlin v Union Saint-Gilloise

Sebastian Haller felt Signal Iduna Park was "burning" as he celebrated his first Bundesliga goal for Borussia Dortmund.

The 28-year-old netted in a symbolic moment, with his maiden goal in the 5-1 rout of Freiburg coming on World Cancer Day.

After signing for Dortmund in July, Haller was diagnosed with testicular cancer and did not feature in the first half of the season during his recovery.

A long-awaited debut came in January, and he got onto the scoresheet on Saturday.

Haller's goal resulted in rapturous celebrations from the home fans, who had seen their club promote awareness to testicular cancer on the day, which delighted the Ivory Coast forward.

"Scoring my first goal means a lot to me," he told Sky.

"When I scored the goal, I felt that the whole stadium was burning. Not only the fans, but also the team-mates and the staff.

"I had been looking forward to this goal from the very first moment."

Dortmund's victory puts them level on points with Bayern Munich in the Bundesliga, who play Wolfsburg on Sunday, sitting two points behind leaders Union Berlin.

Christian Gunter was lost for words after his Freiburg side were thumped 6-0 by Wolfsburg on their return to Bundesliga action on Saturday.

Freiburg entered the two-month break second only to Bayern Munich in the table after winning four of their final five league games of 2022.

But any thoughts of possibly rivalling 10-in-a-row champions Bayern were put into perspective by Wolfsburg at the Volkswagen Arena.

Christian Streich's side were behind after just 59 seconds through a Patrick Wimmer strike, before Jonas Wind's double put the hosts in complete control before half-time.

Yannick Gerhardt and Ridle Baku joined the scoresheet in the second half, and Luca Waldschmidt beat Mark Flekken from the penalty spot to complete the scoring.

"I don't have any explanation. It wasn't expected. We have to look at what went wrong," Freiburg captain Gunter told Sky Sport.

"It's extremely painful to start like this in 2023. Everything came together for them; it was a very bad day for us."

Freiburg's heavy loss sees them drop to fourth below Eintracht Frankfurt and Union Berlin, with all three sides five points behind leaders Bayern.

Reflecting on a chastening day for his side, Freiburg head coach Christian Streich said: "We lost 6-0 and didn't have a chance.

"After the first minute, we got to know how good Wolfsburg are. In the end, we lost 6-0. What can I say? Maybe it's best I don't say anything."

Wolfsburg's win was their fifth in a row in Bundesliga – their best run since 2014 when winning six in a row – and moves them within four points of the Champions League spots.

"We got off to a very good start," Wolfsburg coach Niko Kovac said. "From our second shot, we scored our second goal, then it was a sure-fire success from there.

"Basically, you can't go wrong from there. I'm happy with the way this team is developing."