Anett Kontaveit and Maria Sakkari were both knocked out of the Ostrava Open on Thursday as several top seeds tumbled.
Third seed Kontaveit, who won this competition last year, retired when a set down to Tereza Martincova, while world number seven Maria Sakkari was defeated 5-7 7-5 7-5 by 21-year-old American Alycia Parks in a big upset.
Parks lost the opener but found two crucial breaks of serve when 6-5 up in both the second and third sets to eliminate the fourth seed.
The withdrawal of Belinda Bencica – seeded sixth – with a foot injury put Barbora Krejcikova into the quarter-finals, while Ekaterina Alexandrova will also be in the last eight after overcoming fifth seed Daria Kasatkina 6-1 7-6 (7-1).
The tournament favourites were in much better form at the Jasmin Open in Tunisia, as the top two seeds sealed their places in the quarter-finals with straight-sets wins.
Favourite Ons Jabeur cruised to a 6-1 6-3 victory over Evgeniya Rodina, converting four of her seven break points to safely advance to the next round of her home tournament.
And number two seed Veronika Kudermetova eased past Magdalena Frech 6-3 6-4 to set up a quarter-final meeting with Diane Parry.