Jonas Wind's extra-time winner saw Wolfsburg dump Borussia Dortmund out of the DFB-Pokal in the second round, piling more pressure on Nuri Sahin.
With the game goalless after 90 minutes, the encounter at the Volkswagen Arena looked destined for penalties until Wind tapped home from close range in the 117th minute.
Dortmund started strongly and almost took the lead early on when Maximilian Beier was sent through on goal, but struck the post with only Kamil Grabara to beat.
Further chances fell to Serhou Guirassy and Beier, but the visitors were unable to take advantage of their dominant first 45 minutes.
Wolfsburg grew into the contest after the break, and came closest in the second minute of extra-time when Tiago Thomas hit the woodwork before Wind had a header cleared off the line by Ramy Bensebaini.
But Denmark international Wind would have the final say, netting the winner to leave Dortmund winless in their last three games in all competitions.
It was, however, a more comfortable evening for holders Bayer Leverkusen, who swept aside second-tier Elversberg 3-0 on Tuesday.
Patrik Schick scored a brace in the first nine minutes to put Xabi Alonso's side in control, with Aleix Garcia adding Leverkusen's third with a stunning free-kick before the break.
Elversberg beat Leverkusen in the first round of the 2022-23 DFB-Pokal, making them the last team to beat the current holders in the competition.
RB Leipzig, the winners of back-to-back DFB-Pokal's before Leverkusen's triumph last year, also went into the hat for the next round after a 4-2 win over St. Pauli.
Yussuf Poulsen and Christoph Baumgartner handed Marco Rose's side an early advantage before Morgan Guilavogui halved the deficit at the Red Bull Arena.
Poulsen notched his second on the half-hour mark, only for Eric Smith to score the visitors' second after the interval.
But Leipzig's progression was sealed by Antonio Nusa, who rounded off a fine solo run with his third goal for the club in all competitions.