The unbeaten Steelers confirmed on Tuesday that quarterback Roethlisberger, offensive lineman Jerald Hawkins, running back Jaylen Samuels and linebacker Vince Williams are isolating for at least five days.
The quartet are permitted to take part in virtual meetings and must test negative before they can be considered for Sunday's game against the Cincinnati Bengals at Heinz Field.
The announcement was made a day after tight end Vance McDonald was placed on the list after testing positive for the virus.
The Steelers defeated the Dallas Cowboys 24-19 to move to 8-0 at the top of the AFC North on Sunday.
Veteran quarterback Roethlisberger, offensive lineman Jerald Hawkins, running back Jaylen Samuels and linebacker Vince Williams were added to the list on Tuesday.
However, the quartet are now able to face the Bengals at Heinz Field on Sunday, though tight end Vance McDonald remains sidelined following a positive COVID-19 test.
The Steelers are 8-0 and go into Week 10 as the only unbeaten team left in the NFL. Had Roethlisberger been ineligible, Pittsburgh would have become the first team since the Denver Broncos in 1998 to change their starting quarterback with an unbeaten record and at least eight or more wins.
Roethlisberger has thrown for 1,934 yards with 18 touchdowns and four interceptions this season.
He will be up against Bengals rookie Joe Burrow, who has passed 330 times, has five 300-yard games and averages 284.0 yards per contest with a record of 2-5-1 after eight starts in the league.
At the same point in his debut season in 2004, Roethlisberger had attempted 172 passes, had no 300-yard games and averaged 176.5 yards game. However, the Steelers were 8-0.
