The Bahamian produced a world-leading 31.78 to win the men’s 300m ahead of American Matthew Boling (32.58) and British World Championship silver medallist Matthew Hudson-Smith (33.82).
The 28-year-old's time was also the second fastest ever indoors, only trailing his 31.56 done at the same venue in 2022.
Gardiner, who also took gold at the World Championships in Doha in 2019, was on his way to another undefeated season in the 400m before pulling up with an injury in the semi-finals at the World Championships in Budapest last August.
In the women’s equivalent in South Carolina, Jamaican Charokee Young ran 37.38 for second behind American Quanera Hayes who won in an excellent 36.36. Tierra Robinson-Jones was third in 38.44.
Two-time Jamaican national 200m champion Andrew Hudson ran 6.74 to take top spot in the men’s 60m ahead of Miles Stephens (6.89) and Doniven Jackson (6.92).
In the field, Guyanese Limestone College senior Lloyd McCurdy jumped 14.50m to win the men’s triple jump ahead of Wingate’s Dequan Thompson (14.44m) and Limestone’s Trevon Jenkins (14.18m).
Guyana's Lloyd McCurdy and Jamaican Sadiki Marsh were among the winners on the men’s side.
The 23-year-old McCurdy, competing unattached, jumped a personal best 16.04m to take the win ahead of Jamaicans Shemar Miller, who did 15.27m, and Rajaun Ricketts who did 14.99m. Both Miller and Ricketts attend Benedict College.
Marsh, also a student at Benedict College, won the 800m in 1:55.86 ahead of the Wingate University pair of Ben Aris (1:57.53) and Jakob Rettschlag (1:57.80).
Trinidadian Limestone College senior Che’ Lara ran a personal best 47.68 for second in the 400m behind South Carolina’s William Spencer Jr who ran 47.38 for the win. South Carolina’s Edward Richardson was third in 48.37 while Jamaican St. Augustine’s sophomore Sean Kalawan was fifth 48.96.
Lara’s Limestone College teammate, Grenadian Kurt Modeste, ran 21.33 for third in the 200m behind South Carolina’s Evan Miller (20.95) and Lenoir-Rhyne’s Trent Davis (21.23).
On the women’s side, Haiti’s Mulern Jean sped to 8.27 to win the 60m hurdles. Barbados’ Tia-Adana Belle was second in 8.40 while Jamaican Charleston Southern senior Chaneal Harris was third in 8.55.
Belle also enjoyed a top-three finish in the 200m, finishing third in 24.38 behind 17-year-old American sensation Shawnti Jackson (22.91) and Charleston Southern’s Lauryn James (24.16).
Dominica’s Mariah Toussaint was also a winner on the day, jumping 6.20m to comfortably win the long jump ahead of the University of West Georgia’s Pashience Collier (5.71m) and William Carey’s Zaniyah Wilson (5.68m).