Teams will be allowed to make five substitutions from nine players on the bench for the EFL Cup semi-finals and final.
The change in the rule was introduced following consultation with Manchester United, Manchester City, Tottenham and Brentford, the four semi-finalists in the 2020-21 competition.
VAR will also be introduced, having not been involved in previous rounds.
The EFL added in a statement on Monday: "Each team is only permitted a maximum of three opportunities (excluding half time, full time and half time of extra time) to make substitutions during normal time. However, if a tie goes to extra time, an additional opportunity to use a substitute(s) will also be permitted but only from the five substitutes allowed."
The decision comes despite Premier League clubs voting against the option of using five substitutes in a single game in England's top flight, a rule which has been adopted in other major competitions across Europe this season.
City boss Pep Guardiola has been among the more vocal in his criticism of not continuing the five-sub rule, which was temporarily introduced into the Premier League following the resumption of the 2019-20 season after the coronavirus-enforced shutdown.
City face United at Old Trafford on Wednesday, with Spurs hosting Brentford 24 hours earlier.
This season's semi-finals will be one-legged and go to extra time and penalties if required, rather than the traditional two-legged ties, due to the congested fixture schedule caused by the disruption to the football calendar.
The final was moved from February to April, when it is hoped some fans may be allowed to attend.
Man City star Foden scoops Premier League Player of the Year award
Phil Foden has been named the Premier League's Player of the Year, having been sensational for Manchester City this season.
Foden has scored 17 goals and provided a further eight assists in the top flight in 2023-24.
The 23-year-old has been pivotal in City's quest to win an unprecedented fourth Premier League title.
Pep Guaridola's team head into their final game of the season, in which they face West Ham at home, needing a win to guarantee the crown.
Only six players have bettered Foden's goal tally, with the attacker enjoying his most profitable scoring season for City.
Foden's 17 goals have come from an expected goals figure of 10, suggesting his finishing has been way above what would have been anticipated based on the quality of chances he has had.
The England international, meanwhile, has had 99 shots, and leads City's squad for chances created (68).
"To win this award is an achievement that I am extremely proud of," Foden told Man City's official website.
"Overall, I've been very happy with the way I have played this season and really pleased that I have been able to contribute with the goals and assists throughout the season.
"I'd like to thank all of the City staff, coaches and especially my teammates because without them this wouldn’t be possible.
"And I would also like to take this opportunity to thank everyone who voted for me as the award means a lot."
It is the fifth season in a row that a City player has won the award, with Kevin De Bruyne (2019-20, 2021-22), Ruben Dias (2020-21) and Erling Haaland (2022-23) having scooped it previously.
Fiorentina 2-2 Napoli: Kvaratskhelia keeps visitors' slim European hopes alive
Dethroned Italian champions Napoli's hopes of playing European football next season are hanging by a thread after they played out a 2-2 draw at Fiorentina in Serie A on Friday.
Napoli took the lead in the eighth minute through defender Amir Rrahmani, who nodded home a corner by Matteo Politano.
Cristiano Biraghi levelled for Fiorentina in the 40th minute from a free-kick, before M'Bala Nzola put the hosts in front two minutes later when he fired a shot into the bottom corner of the net.
Napoli's Khvicha Kvaratskhelia responded with another stunning free-kick early in the second half to rescue a point for his team.
Eighth-placed Fiorentina, on 54 points and with a game in hand, are five points behind Lazio in seventh.
They can still earn a Europa League spot either by leapfrogging the Rome side or by winning the Europa Conference League final in which they face Greece's Olympiakos on May 29.
Ninth-placed Napoli, on 52 points, still have a slim chance of making the Europa Conference League playoffs.
Data Debrief
Fiorentina left-back Biraghi has now scored six goals from direct free-kicks since the start of 2021-22, which is behind only James Ward-Prowse (seven) - now of West Ham and formerly of Southampton - as the most across Europe's top five leagues in that period.
Kvaratskhelia also levelled via a direct free-kick, making this the first Serie A match in which both teams scored via that route since Fiorentina against Lazio in April 2018 (Jordan Veretout and Luis Alberto in that case).
Southampton 3-1 West Brom (3-1 agg): Saints set up play-off final with Leeds United
Southampton saw off West Brom 3-1 at St Mary's on Friday to set up a Championship play-off final showdown with Leeds United.
Leeds thumped Norwich City 4-0 at Elland Road 24 hours earlier to book their place at Wembley, where Southampton now await on May 26.
The first leg between the Saints and West Brom finished goalless - just like the other semi-final - but the home side's quality told in the end.
Russell Martin's side dominated the first-half possession, with David Brooks going closest to scoring with a shot that hit the outside of the post.
Southampton made the breakthrough via a powerful Will Smallbone finish four minutes into the second half after Gray Diangana lost possession.
Darnell Furlong had a shot helped over by Alex McCarthy, while David Brooks - who assisted the opener - had a strong penalty appeal rejected.
Southampton remained in control, though, and Adam Armstrong scored a second with a shot through Furlong's legs and past Alex Palmer.
Armstrong's second goal arrived from the penalty spot in the 86th minute after Tom Fellows fouled Ryan Manning in the box, rendering Cedric Kipre's late header a mere consolation.
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