Belgium star Romelu Lukaku will miss the Nations League clash with Poland due to injury as Roberto Martinez implored his side to respond from defeat to Netherlands.
Martinez's side are ranked second in the world but were thoroughly outplayed on Friday as Netherlands cruised to a 4-1 victory.
Lukaku limped off in the first half with the scores level, before goals from Steven Bergwijn, Denzel Dumfries and Memphis Depay secured Netherlands' first win on Belgian turf since 1997.
Martinez assured the issue with Lukaku was simply "a knock" after the game, but Belgium confirmed on Monday that the Chelsea striker will be absent for the clash with Poland on Wednesday.
The Red Devils added Lukaku has started treatment, leaving it unclear whether he will be fit to feature for the remaining clashes in June against Wales and the reverse fixture with Poland.
While Martinez may be without his talisman as Belgium ramp up their World Cup preparations for Qatar, the Red Devils coach urged his team to respond against Poland.
"[The Netherlands defeat] reminded us of the level we have to reach for the World Cup," he told reporters.
"This type of tough game will help us improve. We don't like to lose but we'll learn. It's an ongoing process, and the next test will come quickly against Poland."
The defeat to Netherland was also the first time Belgium had lost on home soil against any side since September 2017, while it was the first time the Red Devils had conceded four goals at home since October 2010.
Poland visit Brussels in the next League A Group 4 fixture and coach Czeslaw Michniewicz expects Belgium to come out fighting for a response.
"The Netherlands [won 4-1 in] Belgium, but both teams could have scored more; the Belgians had a lot of chances, plenty of set-pieces," Michniewicz said.
"We will have an interesting meeting with a team that has many classy individuals. Belgium will be motivated after such a heavy defeat."
Borussia Dortmund 4-0 Darmstadt: Reus stars in farewell game
Marco Reus scored and assisted in his final Bundesliga game for Borussia Dortmund as they thrashed already-relegated Darmstadt 4-0.
The German teed up Ian Maatsen for the opener on the half-hour mark before scoring a stunning free-kick eight minutes later to put Dortmund in control.
Donyell Malen scored his 13th goal of the season to round things off after substitute Julian Brandt had latched onto Jadon Sancho's ball for a third.
Dortmund could have had more as Reus, Malen and Marcel Sabitzer struck the woodwork in either half, but it did not matter as Darmstadt struggled to show a threat.
Edin Terzic's side finished fifth in the final Champions League spot, while Darmstadt are relegated as the bottom club.
Data Debrief:
Reus has been directly involved in 251 goals in the Bundesliga (156G 95A in 391 games), making him just the third player to reach 250 goals and assists since his Bundesliga debut in August 2009, behind Thomas Müller 318 and Robert Lewandowski 367.
The German bids farewell to Signal Iduna Park having scored nine goals and assisting seven in all competitions this season, though he will be hoping to add to that tally in his final match for Dortmund - the Champions League final against Real Madrid on June 1.
Chelsea edge out Man City to claim fifth straight WSL title with thumping win at Old Trafford
Mayra Ramirez turned in a stellar performance to propel Chelsea to the WSL title in Emma Hayes' final match in charge as the Blues thrashed Manchester United 6-0.
Earlier in May, Hayes – who is ending a 12-year stint in charge of the Blues to become the United States coach – had all but given up on claiming a fifth straight WSL crown.
Yet Chelsea's 8-0 victory over Bristol City two weeks ago, combined with Manchester City's defeat to Arsenal, opened the door, and after beating Tottenham in midweek, the Blues knew a win, while maintaining their positive goal difference, would be enough.
And they made sure there was no room for error with an incredible first-half display inspired by Ramirez, who scored twice and set up goals for Johanna Rytting Kaneryd and Sjoeke Nusken at Old Trafford.
Melanie Leupolz got in on the act early in the second half as Chelsea's celebrations began in earnest, with Fran Kirby netting late on to complete the rout on her farewell appearance.
Ultimately, City – likely deflated by the news coming from Old Trafford – held up their end of the bargain, but shorn of top goalscorer Khadija Shaw, Gareth Taylor's team could only beat Aston Villa 2-1.
City were constantly chasing their title rivals, with Ramirez and Rytting Kaneryd putting Chelsea 2-0 up at United inside eight minutes.
Mary Fowler nudged City ahead at Villa Park, though when Taylor's team went in at the interval, they will have seen additional goals from record signing Ramirez and Nusken had all but sewn up the title for Chelsea already.
Leupolz has scored her first WSL goal in over two years to add further gloss for Chelsea when they came back out from the restart in Manchester, and City were then hit by a further blow when Rachel Daly equalised for Villa.
Lauren Hemp, who had assisted Fowler's opener, restored City's lead, though it was never going to be enough, with Kirby's farewell goal ensuring Chelsea finished with a goal difference of 53 compared to their rivals' 46.
Chelsea are now unbeaten across their last nine WSL games that have come on the final day of the season, while the Blues are now nine WSL matches without loss against United, having won eight of those fixtures.
Hoffenheim 4-2 Bayern Munich: Tuchel bows out on defeat thanks to Kramaric hat-trick
Bayern Munich squandered a two-goal lead to slump to a 4-2 loss at Hoffenheim courtesy of a second-half Andrej Kramaric hat-trick in Thomas Tuchel's final game.
Early goals from Mathys Tel and Alphonso Davies saw the injury-depleted Bavarians go 2-0 up by the sixth minute.
Hoffenheim, however, pulled a goal back through Germany international Maximilian Beier just two minutes later, with the hosts gradually taking some of the early pace out of the game.
Bayern's injury troubles were further compounded when they lost Aleksandar Pavlovic to injury in the 35th minute, and struggled to cope with Hoffenheim's threat in the second half.
Kramaric then delivered a stunning 19-minute hat-trick with the Bayern defence in ruins, ending a disappointing season in third place with 72 points.
Bayer Leverkusen finished in top spot on 90 points. VfB Stuttgart finished runners-up on 73 with a 4-0 demolition of Borussia Moenchengladbach, while Hoffenheim finished seventh, in the Europa Conference League place.
Data Debrief:
Thomas Muller made his 473rd Bundesliga appearance for Bayern on Saturday, drawing level with Sepp Maier as the club's record appearance maker in the competition.
Bayern failed to have a single shot on target in the second half, while Kramaric ran riot to subject Bayern to their worst league finish since 2011.
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